1. 重组蛋白
  2. Enzymes & Regulators
  3. Matrix Metalloproteinases
  4. MMP-9
  5. MMP-9 蛋白, Human (HEK293, His, solution)

MMP-9 蛋白, Human (HEK293, His, solution)

目录号: HY-P70145
COA 产品使用指南

MMP-9 蛋白是一种基质金属蛋白酶,对于局部细胞外基质蛋白水解和白细胞迁移至关重要。提示其可能参与骨破骨细胞吸收。MMP-9 蛋白, Human (HEK293, His, solution) 是重组的 MMP-9 蛋白,由 HEK293 表达,带有 C-6*His 标签。

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规格 价格 是否有货 数量
10 μg ¥1654 In-stock
50 μg ¥4950 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The MMP-9 protein is a matrix metalloproteinase that is critical for local extracellular matrix proteolysis and leukocyte migration. It is suggested that it may be involved in bone osteoclastic resorption. MMP-9 Protein, Human (HEK293, His, solution) is the recombinant human-derived MMP-9 protein, expressed by HEK293 , with C-6*His labeled tag.

研究背景

MMP-9 蛋白是一种基质金属蛋白酶,在细胞外基质的局部蛋白水解中发挥着至关重要的作用,并促进白细胞迁移。它可能参与骨破骨细胞吸收并在特定的 Gly-|-Leu 键处裂解 KiSS1。此外,MMP-9 裂解 NINJ1 以生成分泌型 ninjurin-1 形式,并将 IV 型和 V 型胶原加工成较大的 C 端四分之三片段和较短的 N 端四分之一片段。在降解纤连蛋白时,MMP-9 不会影响层粘连蛋白或 Pz 肽,显示出其在底物裂解中的选择性。

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate 20 μM Mca-PLGL-Dpa-AR-NH2(HY-131498)that incubate at room temperature in kinetic mode for 5 minutes.The specific activity is ≥80000 pmol/min/µg.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAH06093.1 (A19-D707)

基因 ID
蛋白结构
N-term
MMP-9 (A19-D707)
Accession # AAH06093.1
6*His
C-term
同用名
rHuMatrix metalloproteinase-9/MMP-9, His; Matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; MMP9
氨基酸序列

AAPRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGVVVPTRFGNADGAACHFPFIFEGRSYSACTTDGRSDGLPWCSTTANYDTDDRFGFCPSERLYTRDGNADGKPCQFPFIFQGQSYSACTTDGRSDGYRWCATTANYDRDKLFGFCPTRADSTVMGGNSAGELCVFPFTFLGKEYSTCTSEGRGDGRLWCATTSNFDSDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMYPMYRFTEGPPLHKDDVNGIRHLYGPRPEPEPRPPTTTTPQPTAPPTVCPTGPPTVHPSERPTAGPTGPPSAGPTGPPTAGPSTATTVPLSPVDDACNVNIFDAIAEIGNQLYLFKDGKYWRFSEGRGSRPQGPFLIADKWPALPRKLDSVFEEPLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPED

分子量

Approximately 90.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 2 mM CaCl2, 150 mM NaCl, 0.05% Brij35(w/v), pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

MMP-9 Protein, Human (HEK293, His, solution) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MMP-9 Protein, Human (HEK293, His, solution)
目录号:
HY-P70145
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