1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. MRCKβ 蛋白, Human (sf9, GST)

MRCKβ 蛋白, Human (sf9, GST)

目录号: HY-P702635
产品使用指南

CDC42BPB 蛋白是 CDC42 下游的关键丝氨酸/苏氨酸蛋白激酶,控制细胞骨架动力学和细胞迁移。CDC42BPB 磷酸化 PPP1R12C 和 MYL9/MLC2,调节肌动蛋白重组并塑造细胞动力学。MRCKβ 蛋白, Human (sf9, GST) 是重组的 MRCKβ 蛋白,由 Sf9 insect cells 表达,带有 GST 标签。MRCKβ 蛋白, Human (sf9, GST)

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描述

CDC42BPB protein is a key serine/threonine protein kinase downstream of CDC42 that controls cytoskeletal dynamics and cell migration. CDC42BPB phosphorylates PPP1R12C and MYL9/MLC2, regulates actin reorganization and shapes cell dynamics. MRCKβ Protein, Human (sf9, GST) is the recombinant human-derived MRCKβ, expressed by Sf9 insect cells , with GST labeled tag. ,

研究背景

CDC42BPB 蛋白是一种丝氨酸/苏氨酸蛋白激酶,是 CDC42 的关键下游效应子,对细胞骨架重组和细胞迁移产生重大影响。通过 PPP1R12C 和 MYL9/MLC2 的磷酸化,CDC42BPB 协调肌动蛋白细胞骨架重组的调节,强调其在塑造细胞动力学中的作用。CDC42BPB 与 MYO18A 和 LURAP1 合作,积极参与层状肌动球蛋白逆行流的调节,这是细胞突出和迁移的关键过程。此外,CDC42BPB 磷酸化 PPP1R12A,从而对细胞功能产生多方面的影响。CDC42BPB 与 FAM89B/LRAP25 协同作用,促进 LIMK1 靶向片状足,启动其激活和随后的 CFL1 磷酸化,这是调节片状足 F-肌动蛋白动力学的关键事件。

种属

Human

表达系统

Sf9 insect cells

标签

GST

蛋白编号

Q9Y5S2 (S2-G473)

基因 ID

9578

同用名
CDC42BPB; CDC42 binding protein kinase beta (DMPK-like); MRCKB; KIAA1124; CDC42-binding protein kinase beta; myotonic dystrophy protein kinase-like beta; CDC42-binding protein kinase beta (DMPK-like); EC 2.7.11.1; DMPK-like beta; MRCK beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; Serine/threonine-protein kinase MRCK beta
纯度

Greater than 80 % as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MRCKβ Protein, Human (sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MRCKβ Protein, Human (sf9, GST)
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