MRGPRX2 蛋白, Human (Cell-Free, His)

The MRGPRX2 protein functions as a mast cell-specific receptor that responds to basic secretagogues and peptides with a cyclic tetrahydroisoquinoline (THIQ) structure. It recognizes ligands such as nonsteroidal neuromuscular blocking drugs (NMBD) and various peptides and alkaloids, including cortistatin-14, proadrenomedullin N-telopeptide PAMP-12, and the antimicrobial protein LL-37. MRGPRX2 Protein, Human (Cell-Free, His) is the recombinant human-derived MRGPRX2 protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of MRGPRX2 Protein, Human (Cell-Free, His) is 330 a.a., with molecular weight of 43.1 kDa.

MRGPRX2 蛋白是一种肥大细胞特异性受体，可作为碱性促分泌剂的受体，包括阳离子两亲性药物和具有基本头基和疏水核心的肽。它识别具有环化四氢异喹啉的小分子，例如管箭毒碱和阿曲库铵等非甾体神经肌肉阻滞药物。结合后，MRGPRX2 会介导以组胺释放、炎症和气道收缩为特征的假过敏反应。此外，它还充当各种配体的受体，包括肽（皮质抑素-14、肾上腺髓质素原 N 端肽 PAMP-12 和 PAMP-20、抗菌蛋白 LL-37、PMX-53 肽和 β-防御素）和生物碱，例如孔雀草定 A.

Human

E. coli Cell-free

N-6*His

Q96LB1 (M1-V330)

117194

MDPTTPAWGTESTTVNGNDQALLLLCGKETLIPVFLILFIALVGLVGNGFVLWLLGFRMRRNAFSVYVLSLAGADFLFLCFQIINCLVYLSNFFCSISINFPSFFTTVMTCAYLAGLSMLSTVSTERCLSVLWPIWYRCRRPRHLSAVVCVLLWALSLLLSILEGKFCGFLFSDGDSGWCQTFDFITAAWLIFLFMVLCGSSLALLVRILCGSRGLPLTRLYLTILLTVLVFLLCGLPFGIQWFLILWIWKDSDVLFCHIHPVSVVLSSLNSSANPIIYFFVGSFRKQWRLQQPILKLALQRALQDIAEVDHSEGCFRQGTPEMSRSSLV

Monomer:37 kDa Dimer: 74 kDa It is speculated that the protein forms a dimeric structure.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6%Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.