1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. MSRB3 蛋白, Human (HEK293, His)

MSRB3 蛋白, Human (HEK293, His)

目录号: HY-P76501
COA 产品使用指南

MSRB3 蛋白具有独特的催化蛋氨酸亚砜还原的能力,在听觉系统中发挥着至关重要的作用。MSRB3 亚型 2 被认为对于正常听力至关重要,凸显了其在听觉功能中的重要性。MSRB3 蛋白, Human (HEK293, His) 是重组的 MSRB3 蛋白,由 HEK293 表达,带有 C-His 标签。MSRB3 蛋白, Human (HEK293, His) 全长 156 个氨基酸,分子量约为 ~18.4 KDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

MSRB3 Protein, with a unique ability to catalyze methionine sulfoxide reduction, plays a crucial role in the auditory system. Isoform 2 of MSRB3 is deemed essential for normal hearing, highlighting its significance in auditory function. MSRB3 Protein, Human (HEK293, His) is the recombinant human-derived MSRB3 protein, expressed by HEK293 , with C-His labeled tag. The total length of MSRB3 Protein, Human (HEK293, His) is 156 a.a., with molecular weight of ~18.4 KDa.

研究背景

MSRB3 是一种蛋白质,具有催化游离和蛋白质结合的甲硫氨酸亚砜还原为甲硫氨酸的独特能力,因其在听觉系统中的关键作用而脱颖而出。具体来说,MSRB3 的异构体 2 被认为对于正常听力至关重要,强调了其在听觉功能中的重要性。

生物活性

Measured by its ability to through oxidizing DTT. The specific activity is 17261.04 pmoL/min/μg, as measured under the described conditions.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q8IXL7-2 (G26-D181)

基因 ID
蛋白结构
N-term
MSRB3 (G26-D181)
Accession # Q8IXL7-2
His
C-term
同用名
Methionine-R-sulfoxide reductase B3; MSRB3
氨基酸序列

GSCRDKKNCKVVFSQQELRKRLTPLQYHVTQEKGTESAFEGEYTHHKDPGIYKCVVCGTPLFKSETKFDSGSGWPSFHDVINSEAITFTDDFSYGMHRVETSCSQCGAHLGHIFDDGPRPTGKRYCINSAALSFTPADSSGTAEGGSGVASPAQAD

分子量

Approximately 19 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MSRB3 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MSRB3 Protein, Human (HEK293, His)
目录号:
HY-P76501
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