MST2 蛋白, Human (Sf9, GST)

MST2 protein is a stress-activated kinase that plays an important role in the Hippo signaling pathway, regulates organ size and inhibits tumors. Cleaved MST2 enters the nucleus and induces apoptosis through chromatin condensation. MST2 Protein, Human (Sf9, GST) is the recombinant human-derived MST2 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of MST2 Protein, Human (Sf9, GST) is 491 a.a., .

MST2 蛋白是一种应激激活的促凋亡激酶，有助于各种细胞过程，是 Hippo 信号通路的关键组成部分，在器官大小控制和肿瘤抑制中发挥核心作用。半胱天冬酶裂解后，MST2 进入细胞核，诱导染色质浓缩和核小体间 DNA 断裂，促进细胞凋亡。在 Hippo 通路中，MST2 与其对应物 STK4/MST1 和调节蛋白 SAV1 一起形成激酶级联，这对于磷酸化和激活 LATS1/2-MOB1 复合物至关重要。该级联进一步磷酸化和灭活 YAP1 癌蛋白和 WWTR1/TAZ，从而调节细胞增殖、死亡和迁移所必需的基因。MST2 和 MST1 对于抑制肝细胞增殖、防止兼性成体肝干细胞的激活以及抑制肿瘤形成至关重要。除了 Hippo 通路之外，MST2 还磷酸化 NKX2-1、NEK2、CROCC、CEP250、MOBKL1A、MOBKL1B 和 RASSF2，对中心体分离、RAF1 激活、ESR1 转录活性和 STK38 激活产生影响。MST2 与 SAV1 协同作用，通过抑制“Ser-259”上的抑制性磷酸化来正向调节 RAF1 激活，并与 MOBKL1B 协作激活 STK38。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The KM of MST2 P3 is 54.85 µM.

Human

Sf9 insect cells

N-GST

Q13188 (M1-F491)

6788

MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF

Approximately 82.8 KDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol 5mM DTT,0.1M Trehalose.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.