1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Growth Differentiation Factor
  5. Growth Differentiation Factor-8 (GDF-8)
  6. MSTN 蛋白, Cat (HEK293, His)

MSTN 蛋白, Cat (HEK293, His)

目录号: HY-P700498
COA 产品使用指南

MSTN(肌肉生长抑制素)作为骨骼肌生长的专用负调节因子,通过二硫键形成同型二聚体。它通过相互作用抑制 WFIKKN2 活性,并通过与 FSTL3 结合进一步发挥其负调节骨骼肌生长的作用。MSTN 蛋白, Cat (HEK293, His) 是重组的 MSTN 蛋白,由 HEK293 表达,带有 N-10*His 标签。MSTN 蛋白, Cat (HEK293, His) 全长 357 个氨基酸,分子量为 46 kDa。

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
20 μg ¥2650 In-stock
50 μg ¥5000 In-stock
100 μg ¥8000 In-stock
> 100 μg   询价  

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  • 技术参数

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描述

MSTN (Myostatin) serves as a dedicated negative regulator of skeletal muscle growth, forming homodimers through disulfide linkages. It inhibits WFIKKN2 activity through interaction and further contributes to its role in negatively modulating skeletal muscle growth by engaging with FSTL3. MSTN Protein, Cat (HEK293, His) is the recombinant cat-derived MSTN protein, expressed by HEK293 , with N-10*His labeled tag. The total length of MSTN Protein, Cat (HEK293, His) is 357 a.a., with molecular weight of 46 kDa.

研究背景

MSTN(肌肉生长抑制素)作为骨骼肌生长的专用负调节因子,通过二硫键形成同型二聚体。它与 WFIKKN2 相互作用,有效抑制 WFIKKN2 的活性。此外,MSTN 与 FSTL3 结合,进一步发挥其负调节骨骼肌生长的作用。

种属

Cat

表达系统

HEK293

标签

N-10*His

蛋白编号

M3WPT7 (G19-S375)

基因 ID
蛋白结构
N-term
10*His
MSTN (G19-S375)
Accession # M3WPT7
C-term
同用名
myostatin; GDF8; MSLHP; growth/differentiation factor 8; GDF-8; growth differentiation factor 8
氨基酸序列

GPVDLNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNISKDAIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDLLMQVEGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVKTPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS

分子量

46 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MSTN Protein, Cat (HEK293, His)
目录号:
HY-P700498
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