NAA10/ARD1 蛋白, Human (sf9, His-GST)

NAA10, the catalytic subunit of N-terminal acetyltransferase complexes, exhibits alpha (N-terminal) acetyltransferase activity, playing a crucial role in acetylating amino termini lacking initiator methionine. This activity holds significance in processes related to vascular, hematopoietic, and neuronal growth and development. In conjunction with NAA15, NAA10 displays epsilon (internal) acetyltransferase activity towards HIF1A, leading to its degradation and impacting cellular responses. Notably, NAA10 has diverse roles, repressing MYLK kinase activity through acetylation to inhibit tumor cell migration and stabilizing TSC2, thereby suppressing mTOR activity and impeding cancer development. Furthermore, NAA10 acetylates HSPA1A and HSPA1B at 'Lys-77,' enhancing their chaperone activity and promoting preferential binding to co-chaperone HOPX. Additional substrates include HIST1H4A, indicating a broad impact on cellular processes. Intriguingly, NAA10 acts as a negative regulator of sister chromatid cohesion during mitosis, underscoring its multifunctional role in cellular homeostasis. NAA10/ARD1 Protein, Human (sf9, His-GST) is the recombinant human-derived NAA10/ARD1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of NAA10/ARD1 Protein, Human (sf9, His-GST) is 235 a.a., with molecular weight of ~49 kDa.

NAA10 是 N 末端乙酰转移酶复合物的催化亚基，具有 α（N 末端）乙酰转移酶活性，在乙酰化缺乏引发剂蛋氨酸的氨基末端中发挥着至关重要的作用。这种活性在与血管、造血和神经元生长和发育相关的过程中具有重要意义。 NAA10 与 NAA15 结合，对 HIF1A 表现出 epsilon（内部）乙酰转移酶活性，导致其降解并影响细胞反应。值得注意的是，NAA10 具有多种作用，通过乙酰化抑制 MYLK 激酶活性，从而抑制肿瘤细胞迁移并稳定 TSC2，从而抑制 mTOR 活性并阻止癌症发展。此外，NAA10 在“Lys-77”处乙酰化 HSPA1A 和 HSPA1B，增强其伴侣活性并促进与辅助伴侣 HOPX 的优先结合。其他底物包括 HIST1H4A，表明对细胞过程具有广泛影响。有趣的是，NAA10 在有丝分裂过程中充当姐妹染色单体凝聚力的负调节因子，强调了其在细胞稳态中的多功能作用。

Human

Sf9 insect cells

N-His;N-GST

P41227 (M1-S235)

8260  [NCBI]

Approximately 49 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.