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  3. NAA10/ARD1 蛋白, Human (sf9)

NAA10/ARD1 蛋白, Human (sf9)

目录号: HY-P77096
产品使用指南

NAA10/ARD1 蛋白是 N 末端乙酰转移酶复合物的催化亚基,具有 α 乙酰转移酶活性。NAA10/ARD1 影响血管、造血和神经元的生长和发育。NAA10/ARD1 抑制肿瘤细胞迁移、抑制 mTOR 活性和癌症发展。NAA10/ARD1 蛋白, Human (sf9) 是重组的 NAA10/ARD1 蛋白,由 Sf9 insect cells 表达,不带标签。NAA10/ARD1 蛋白, Human (sf9) 全长 235 个氨基酸,分子量约为 ~31 kDa。

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描述

The NAA10/ARD1 protein is the catalytic subunit of the N-terminal acetyltransferase complex and has alpha acetyltransferase activity. NAA10/ARD1 affects vascular, hematopoietic, and neuronal growth and development. NAA10/ARD1 inhibits tumor cell migration, inhibits mTOR activity and cancer development. NAA10/ARD1 Protein, Human (sf9) is the recombinant human-derived NAA10/ARD1 protein, expressed by Sf9 insect cells , with tag free. The total length of NAA10/ARD1 Protein, Human (sf9) is 235 a.a., with molecular weight of ~31 kDa.

研究背景

NAA10 是 N 末端乙酰转移酶复合物的催化亚基,具有 α(N 末端)乙酰转移酶活性,在乙酰化缺乏引发剂蛋氨酸的氨基末端中发挥着至关重要的作用。这种活性在与血管、造血和神经元生长和发育相关的过程中具有重要意义。NAA10 与 NAA15 结合,对 HIF1A 表现出 epsilon(内部)乙酰转移酶活性,导致其降解并影响细胞反应。值得注意的是,NAA10 具有多种作用,通过乙酰化抑制 MYLK 激酶活性,从而抑制肿瘤细胞迁移并稳定 TSC2,从而抑制 mTOR 活性并阻止癌症发展。此外,NAA10 在“Lys-77”处乙酰化 HSPA1A 和 HSPA1B,增强其伴侣活性并促进与辅助伴侣 HOPX 的优先结合。其他底物包括 HIST1H4A,表明对细胞过程具有广泛影响。有趣的是,NAA10 在有丝分裂过程中充当姐妹染色单体凝聚力的负调节因子,强调了其在细胞稳态中的多功能作用。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

P41227 (M1-S235)

基因 ID
蛋白结构
N-term
NAA10 (M1-S235)
Accession # P41227
C-term
同用名
N-alpha-acetyltransferase 10; hARD1; NatA catalytic subunit Naa10; ARD1; ARD1A; TE2
分子量

Approximately 31 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NAA10/ARD1 Protein, Human (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NAA10/ARD1 Protein, Human (sf9)
目录号:
HY-P77096
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