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  3. NAD(P)H-flavin reductase 蛋白, E.coli (His)

NAD(P)H-flavin reductase 蛋白, E.coli (His)

目录号: HY-P71709
产品使用指南

NAD(P)H-黄素还原酶蛋白通过使用还原的吡啶核苷酸催化可溶性黄素的还原,在细胞氧化还原过程中发挥关键作用。这种酶活性反映了其将电子从 NAD(P)H 转移到黄素的能力,参与重要的细胞内氧化还原反应。NAD (P) H-flavin reductase 蛋白, E.coli (His) 是重组的 NAD (P) H-黄素还原酶蛋白,由 E. coli 表达,带有 N-His 标签。

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描述

The NAD(P)H-flavin reductase protein plays a key role in cellular redox processes by catalyzing the reduction of soluble flavins using reduced pyridine nucleotides. This enzyme activity reflects its ability to transfer electrons from NAD(P)H to flavin, participating in important intracellular redox reactions. NAD (P) H-flavin reductase Protein, E.coli (His) is the recombinant E. coli-derived NAD(P)H-flavin reductase protein, expressed by E. coli , with N-His labeled tag.

研究背景

NAD(P)H-黄素还原酶蛋白是一种使用还原的吡啶核苷酸(例如NADH或NADPH)催化可溶性黄素还原的酶。这种酶活性对于维持细胞氧化还原平衡至关重要,并参与黄素辅酶作为电子载体的各种代谢过程。通过利用还原的吡啶核苷酸,NAD(P)H-黄素还原酶在将电子转移到黄素方面发挥着关键作用,从而有助于调节细胞氧化应激和氧化还原状态。这种酶参与黄素还原,强调了它在多种生物途径中的重要性,并强调了它在调节细胞对氧化条件的反应中的作用。参考资料:所提供的信息基于截至 2022 年 1 月的一般知识,未引用具体参考资料。

种属

E.coli

表达系统

E. coli

标签

N-His

蛋白编号

P0AEN1 (T2-I233)

基因 ID

58462182  [NCBI]

蛋白结构
N-term
His
FRE_ECOLI (T2-I233)
Accession # P0AEN1
C-term
同用名
fre; fadI; flrD; fsrC; ubiB; b3844; FMN reductase; Ferrisiderophore reductase C
氨基酸序列

TTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDERDKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDEERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHPGLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSERNAREDRLFGDAFAFI

分子量

Approximately 30.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NAD(P)H-flavin reductase Protein, E.coli (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NAD(P)H-flavin reductase Protein, E.coli (His)
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HY-P71709
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