TFAM 蛋白, Mouse (His, Myc)

TFAM proteins are critical in the regulation of mitochondrial transcription, bind to light chain promoters and are essential for basal mitochondrial DNA transcription.In the initiation complex of TFB2M and POLRMT, TFAM recruits POLRMT, and TFB2M induces structural changes in POLRMT, promoting promoter opening.TFAM Protein, Mouse (His, Myc) is the recombinant mouse-derived TFAM protein, expressed by E.coli , with N-10*His, C-Myc labeled tag.

TFAM 蛋白通过与线粒体轻链启动子结合，在线粒体转录调节中发挥关键作用。作为线粒体转录起始复合体（包括 TFB2M 和 POLRMT）的重要组成部分，TFAM 对于线粒体 DNA 的基础转录至关重要。在这个复合体中，TFAM 将 POLRMT 招募到特定的启动子上，而 TFB2M 诱导 POLRMT 的结构变化，促进启动子打开和捕获 DNA 非模板链。TFAM 对于线粒体 RNA 聚合酶准确有效的启动子识别也是必不可少的。它通过紧邻转录起始位点的上游结合，进一步促进 HSP1 和轻链启动子的转录起始。TFAM 能够通过 HMG 盒解开 DNA 并将线粒体轻链启动子 DNA 弯曲成 U 型转弯形状，因此在维持线粒体 DNA 的正常水平方面发挥着关键作用。此外，它可能有助于组织和压缩线粒体 DNA，并可能在转录激活中发挥作用，或者在精子发生过程中在压缩核 DNA 中发挥结构作用。

Mouse

E. coli

N-10*His;C-Myc

P40630 (S43-H243)

21780

34 kDa

Greater than 90 % as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.