NAMPT 蛋白, Mouse (P.pastoris, His)

NAMPT protein has dual functions of cytokine and adipokine. Due to limited ATP activation, its secreted form lacks enzymatic activity. It catalyzes the condensation of nicotinamide and 5-phosphoribosyl-1-pyrophosphate to produce nicotinamide mononucleotide, which is critical for NAD biosynthesis. NAMPT Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived NAMPT protein, expressed by P. pastoris , with N-10*His labeled tag. The total length of NAMPT Protein, Mouse (P.pastoris, His) is 491 a.a., with molecular weight of 57.9 kDa.

NAMPT 蛋白具有双重功能，既可作为具有免疫调节特性的细胞因子，又可作为具有抗糖尿病特性的脂肪因子。有趣的是，其分泌形式缺乏酶活性，部分原因是由于其水平较低，细胞外空间和血浆中 ATP 的激活有限。从功能上来说，NAMPT 催化烟酰胺与 5-磷酸核糖基-1-焦磷酸的缩合，产生烟酰胺单核苷酸——NAD 生物合成中至关重要的中间体。NAMPT 作为哺乳动物 NAD 生物合成途径中的限速成分，在调节生物钟功能中发挥着关键作用。其 NAMPT 依赖性振荡产生 NAD，通过从 NAD 依赖性 SIRT1 介导的抑制中释放核心时钟成分 CLOCK-BMAL1 异二聚体，控制时钟靶基因表达的振荡。

Mouse

P. pastoris

N-10*His

Q99KQ4 (M1-H491)

59027

MNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNIEQDVAPH

Apparently 62 kDa

Greater than 85% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6%Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.