Nectin-1 蛋白, Rat (HEK293, His)

Nectin-1 Protein, with carbohydrate and virion binding activities, plays pivotal roles in axon guidance, synapse assembly, and viral entry. Located in dendrites, growth cone membranes, and synapses, Nectin-1 is implicated in human conditions like cleft lip and ectodermal dysplasia. Its biased expression, especially in Kidney (RPKM 45.4) and Brain (RPKM 31.3), underscores its significance in diverse physiological contexts. Nectin-1 Protein, Rat (HEK293, His) is the recombinant rat-derived Nectin-1 protein, expressed by HEK293 , with C-His labeled tag.

Nectin-1 蛋白以其碳水化合物结合和病毒粒子结合活性为特征，在轴突引导、突触组装调节和病毒进入宿主细胞中发挥着关键作用。Nectin-1 分布在各种细胞成分中，包括树突、生长锥膜和突触，与唇裂、唇腭裂外胚层发育不良综合征、腭裂和外胚层发育不良等人类疾病有关。这种偏向的表达模式，特别是在肾脏 (RPKM 45.4)、脑 (RPKM 31.3) 和其他九个组织中，强调了其在不同生理环境中的重要性。

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Rat Nectin-1. The ED₅₀ for this effect is 125.7 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Rat Nectin-1.The ED₅₀ for this effect is 125.7 ng/mL.

Rat

HEK293

C-His

F1LNP8/NP_001093946 (Q31-A354)

192183  [NCBI]

QVVQVNDSMYGFIGTDVILHCSFANPLPTVKITQVTWQKASNGSKQNMAIYNPTMGVSVLPPYEKRVEFLRPSFIDGTIRLSHLELEDEGMYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRARKGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHRQSLACIVNYHLDRFRESLTLNVQYEPEVTIEGFDGNWYLQRTDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFRGPINYSLAGTYICEATNPIGTRSGQVEVNITEFPYTPTPEHGRRAGQMPTA

Approximately 50-70 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.