NEDD4L 蛋白, Human (His)

The NEDD4L protein is an E3 ubiquitin protein ligase that regulates multiple signaling pathways, including autophagy, innate immunity, and DNA repair. NEDD4L Protein, Human (His) is the recombinant human-derived NEDD4L protein, expressed by E. coli , with N-6*His labeled tag. The total length of NEDD4L Protein, Human (His) is 974 a.a., .

NEDD4L 是一种 E3 泛素蛋白连接酶，对多种细胞过程进行调节控制，包括自噬、先天免疫和 DNA 修复。它通过诱导 SMAD2 和 TGFBR1 泛素化和蛋白酶体依赖性降解来负调节 TGF-β 信号传导。在自噬的背景下，NEDD4L 通过泛素化和降低 ULK1 或 ASCT2 的细胞水平来下调细胞生长。此外，它还促进各种质膜通道的泛素化和内化，例如 ENaC、Nav1.2、Nav1.3、Nav1.5、Nav1.7、Nav1.8、Kv1.3、Kv7.2、Kv7.3、和 CLC5。NEDD4L 还调节 SGK1、TNK2、BRAT1 和 NTRK1 的蛋白水平，其泛素连接酶活性受 NDFIP1 影响。此外，NEDD4L 在黑素细胞树突形成中发挥着至关重要的作用，有助于 TOR 信号传导调节，并通过催化 TRAF3 的“Lys-29”连接半胱氨酸泛素化、增强“Lys-48”和“Lys-63”参与抗病毒先天免疫。TRAF3 的'-连锁泛素化。

Human

E. coli

N-6*His

Q96PU5 (A2-D975)

23327

Approximately 113.9 KDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.