1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. NIMA Related Kinase 3
  5. NEK3 蛋白, Mouse (sf9, His-GST)

NEK3 蛋白, Mouse (sf9, His-GST)

目录号: HY-P76509
产品使用指南

NEK3 蛋白 通过影响微管影响神经元的形态发生和极性。NEK3 蛋白 调节神经元微管乙酰化。NEK3 蛋白 有助于催乳素介导的 PXN 和 VAV2 磷酸化。NEK3 蛋白, Mouse (sf9, His-GST) 是重组的 NEK3 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。NEK3 蛋白, Mouse (sf9, His-GST) 全长 509 个氨基酸,分子量约为 ~72 KDa。

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描述

NEK3 Protein influences neuronal morphogenesis and polarity through effects on microtubules. NEK3 Protein regulates microtubule acetylation in neurons. NEK3 Protein contributes to prolactin-mediated phosphorylation of PXN and VAV2. NEK3 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived NEK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of NEK3 Protein, Mouse (sf9, His-GST) is 509 a.a., with molecular weight of ~72 KDa.

研究背景

NEK3 编码一个丝氨酸/苏氨酸蛋白激酶 NimA 家族成员。NEK3 蛋白与其他 NimA 家族成员的不同之处在于它不受细胞周期调节,主要在细胞质中发现。Nek3 被催乳素刺激激活,导致 VAV2 鸟嘌呤核苷酸交换因子、桩蛋白磷酸化,并激活 RAC1 gtp 酶[1][2]

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

ABK42288 (M1-A509)

基因 ID

/

蛋白结构
N-term
His-GST
NEK3 (M1-A509)
Accession # ABK42288
C-term
同用名
Serine/threonine-protein kinase Nek3; HSPK 36; NimA-related protein kinase 3
分子量

Approximately 72 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NEK3 Protein, Mouse (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NEK3 Protein, Mouse (sf9, His-GST)
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HY-P76509
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