NEK7 蛋白, Human (His-SUMO)

The NEK7 protein is a key kinase that crucially drives mitotic cell cycle progression by enhancing centrosomal microtubule nucleation, ensuring robust spindle formation, and aiding in cytokinesis. Phosphorylation of EML4 at “Ser-146” promotes chromosome aggregation. NEK7 Protein, Human (His-SUMO) is the recombinant human-derived NEK7 protein, expressed by E. coli , with N-6*His-SUMO labeled tag.

NEK7 蛋白是一种关键激酶，通过促进中心体的微管成核活性、确保稳健的有丝分裂纺锤体形成并促进胞质分裂，在有丝分裂细胞周期进程中发挥着至关重要的作用。其多种功能包括 EML4 在“Ser-146”处的磷酸化，促进有丝分裂期间微管的解离，从而促进有效的染色体会聚。NEK7 通过磷酸化 RPS6KB1 并充当 NLRP3 炎性体组装的重要激活剂，将其影响扩展到有丝分裂事件之外。独立于其激酶活性，NEK7 在微管组织中心内解锁 NLRP3，促进 NLRP3:PYCARD 复合物的形成和 CASP1 的激活。值得注意的是，NEK7 充当细胞开关，确保炎症反应和细胞分裂之间的相互排斥；它与 NEK9 的相互作用可防止与 NLRP3 的相互作用以及有丝分裂过程中炎症小体的激活。这些多方面的功能使 NEK7 成为有丝分裂进展和炎症小体反应中的关键协调者，突显了其在多种细胞过程中的调节意义。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

Q8TDX7-1 (M1-S302)

140609

50 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.