NES 蛋白, Human (P.pastoris, His)

NES is critical for brain and eye development and promotes the disassembly of phosphorylated vimentin intermediate filaments during mitosis. It directs the transport of IF proteins and other factors to daughter cells during progenitor cell division, promoting the survival, renewal, and proliferation of neural progenitor cells. NES Protein, Human (P.pastoris, His) is the recombinant human-derived NES protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of NES Protein, Human (P.pastoris, His) is 249 a.a., with molecular weight of ~38 kDa.

NES 对于大脑和眼睛的发育至关重要，在促进有丝分裂过程中磷酸化波形蛋白中间丝 (IF) 的分解方面发挥着至关重要的作用。该蛋白参与祖细胞分裂过程中 IF 蛋白和其他细胞因子向子细胞的运输和分配，有助于神经祖细胞的存活、更新和有丝分裂原刺激的增殖。在体外，NES 形成同二聚体和同四聚体，并倾向于优先与其他中间丝蛋白（例如波形蛋白和 α-internexin）形成异二聚体。值得注意的是，如果巢蛋白水平不超过 25%，这些异二聚体就会组装成中间丝。此外，NES 与 FHOD3 相互作用，进一步强调了它参与复杂的细胞过程（通过相似性）。

Human

P. pastoris

N-6*His

P48681 (P1321-L1569)

10763  [NCBI]

PPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPL

Approximately 38 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.