Neurofilament light polypeptide/NEFL, Mouse (His)

Neurofilament light chain polypeptide (NEFL) is a part of neurofilaments that, in cooperation with NEFM and NEFH, form the structural basis of neurons.It maintains neuronal caliber and assembles filamentous networks with PRPH and INA.Neurofilament light polypeptide/NEFL, Mouse (His) is the recombinant mouse-derived Neurofilament light polypeptide/NEFL, expressed by E.coli , with N-His labeled tag.

神经丝轻链多肽 (NEFL) 是神经丝的一个组成部分，通常与中间丝蛋白 NEFM 和 NEFH 协同维持神经元口径，形成神经元的结构基础。NEFL 与神经元中间丝蛋白如 PRPH 和 INA 结合，可能参与神经元丝状网络的组装。在体外，NEFL 表现出形成同源二聚体并与 NEFH 或 NEFM 进行异源二聚化，进一步形成异源寡聚体的能力。此外，NEFL 与 INA 形成异二聚体。它的相互作用延伸到调节关系，正如它与 ARHGEF28 和 TRIM2 的关联所证明的那样，强调了塑造神经元结构完整性所涉及的复杂的分子相互作用。

Mouse

E. coli

N-His

P08551 (S2-D543)

18039  [NCBI]

SSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD

Approximately 68 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.