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Neuroligin-1NLGN1 蛋白, Mouse (HEK293, C-His)

目录号: HY-P74704A
COA 产品使用指南

Neuroligin-1 (NLGN1) 蛋白 promotes cell interactions with members of the neuroligin family, affecting synaptic function and signaling. It recruits and aggregates synaptic proteins, promotes synapse formation and specifies excitatory synapses. Neuroligin-1NLGN1 蛋白, Mouse (HEK293, His) is the recombinant mouse-derived Neuroligin-1NLGN1 蛋白, expressed by HEK293 , with C-His labeled tag.

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Accession#Q99K10-1 Neuroligin-1NLGN1 (Q46-S697);C-His

研究背景

Neuroligin-1 (NLGN1) 蛋白是一种细胞表面蛋白,通过与神经毒素家族成员的相互作用积极参与细胞间相互作用。这种蛋白质在突触功能和突触信号传递中发挥着关键作用,可能通过招募和聚集其他突触蛋白质来介导其作用。虽然 NLGN1 可能有助于突触的初始形成,但它并不被认为是这一过程所必需的。体外研究证明其能够触发突触前结构的从头形成,强调其参与兴奋性突触的规范。除了突触动力学之外,NLGN1 对于维持清醒质量以及清醒和睡眠期间大脑皮层活动的正常同步至关重要。其复杂的相互作用涉及神经毒素 NRXN1、NRXN2 和 NRXN3,神经毒素上的硫酸乙酰肝素聚糖修饰促进了结合。此外,NLGN1 与 DLG4/PSD-95 相互作用,并与 AIP1、GOPC、PDZRN3 和 NLGN3 相关,强调其在神经系统发育和突触调节中的多种作用。

生物活性

Measured by its binding ability in a functional ELISA. When recombinant Mouse Neuroligin 1 is immobilized at 10 μg/mL can bind biotinylated human Neurexin 1 beta. The KD is 6.095 nM.

种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

Q99K10-1 (Q46-S697)

基因 ID

192167

蛋白结构
N-term
C-term
同用名
Neuroligin-1; NLGN1; KIAA1070
氨基酸序列

QKLDDVDPLVTTNFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGEHRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYVPTEDGPLTKKHTDDLGDNDGAEDEDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARILATKVGCNVSDTVELVECLQKKPYKELVDQDVQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIVDSDDGVSASDFDFAVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAVATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELVPHLHNLNDISQYTSTTTKVPSTDITLRPTRKNSTPVTSAFPTAKQDDPKQQPSPFSVDQRDYSTELS

分子量

Approximately 80-125 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Neuroligin-1NLGN1 Protein, Mouse (HEK293, C-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Neuroligin-1NLGN1 Protein, Mouse (HEK293, C-His)
目录号:
HY-P74704A
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