NGFR Protein, Mouse (HEK293, His)

Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation^[1]. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells^[3].  NGFR Protein, Mouse (HEK293, His) is a recombinant protein with a C-Terminal His label, It consists of 243 amino acids (M1-N243) and is produced in HEK293 cells.

神经生长因子受体（NGFR）不仅在神经组织中表达，也在非神经元正常细胞和癌细胞中表达，如血管周围细胞、牙髓细胞、淋巴滤泡树突状细胞、口腔粘膜和毛囊的基底上皮、前列腺基底细胞和肌上皮细胞^[1]。
人 NGFR 与小鼠 NGFR 蛋白具有 92.45% aa 序列同一性，与大鼠 NGFR 蛋白具有 92.42% aa 序列同一性。
神经生长因子受体（NGFR）是一种I型跨膜蛋白，是TNFR超家族的典型结构，缺乏内在的催化活性。NGFR 信号传导涉及 NF-kB（Rel/NF-kB 转录因子）的激活和转录因子 c-Jun 激酶 (JNK) 的磷酸化，以及神经酰胺产量的增加，导致基因转录或程序性细胞死亡^[2]。
NGFR 诱导 p53 依赖性细胞凋亡和细胞生长停滞以及抑制肿瘤生长^[3]。低亲和力神经生长因子受体 (NGFR) p75NGFR 在没有神经生长因子 (NGF) 结合的情况下诱导细胞凋亡，但在与 NGF 结合时增强神经存活。NGFR 增强 β-淀粉样肽毒性^[4]。NGFR 信号可诱导随后黑色素瘤抗原的下调，并最终抑制 CTL 激活^[5]。

Measured by its ability to inhibit beta-NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.714-3.772 µg/mL in the presence of 2 ng/mL of recombinant human beta-NGF.

• 
  Measured by its ability to inhibit beta-NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 3.772 µg/mL in the presence of 2 ng/mL of recombinant human beta-NGF, corresponding to a specific activity is 2.686×10² units/mg.

Mouse

HEK293

C-6*His

Q9Z0W1 (K32-N253)

18053  [NCBI]

KETCSTGMYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECLGLQSMSAPCVEADDAVCRCSYGYYQDEETGRCEACSVCGVGSGLVFSCQDKQNTVCEECPEGTYSDEANHVDPCLPCTVCEDTERQLRECTPWADAECEEIPGRWITRSTPPEGSDVTTPSTQEPEAPPERDLIASTVADTVTTVMGSSQPVVTRGTADN

Approximately 43-60 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Reis-Filho JS, et al. Distribution and significance of nerve growth factor receptor (NGFR/p75NTR) in normal, benign and malignant breast tissue. Mod Pathol. 2006 Feb;19(2):307-19.  [Content Brief]

  [2]. Micera A, et al. Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate. Cytokine Growth Factor Rev. 2007 Jun-Aug;18(3-4):245-56.  [Content Brief]

  [3]. Zhou X, et al. Nerve growth factor receptor negates the tumor suppressor p53 as a feedback regulator. Elife. 2016 Jun 10;5:e15099.  [Content Brief]

  [4]. Rabizadeh S, et al. Expression of the low-affinity nerve growth factor receptor enhances beta-amyloid peptide toxicity. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10703-6.  [Content Brief]

  [5]. Furuta J, et al. CD271 on melanoma cell is an IFN-γ-inducible immunosuppressive factor that mediates downregulation of melanoma antigens. J Invest Dermatol. 2014 May;134(5):1369-1377.  [Content Brief]

  [6]. Chung MK, et al. CD271 Confers an Invasive and Metastatic Phenotype of Head and Neck Squamous Cell Carcinoma through the Upregulation of Slug. Clin Cancer Res. 2018 Feb 1;24(3):674-683.  [Content Brief]