Niemann Pick C1/NPC1 蛋白, Human (His)

The Niemann Pick C1/NPC1 protein is an intracellular cholesterol transporter that cooperates with NPC2 to promote cholesterol efflux from the endosomal/lysosomal compartment. NPC2 transfers unesterified cholesterol to the N-terminal domain of NPC1, where the cholesterol is bound in a hydrophobic pocket. Niemann Pick C1/NPC1 Protein, Human (His-Flag) is the recombinant human-derived Niemann Pick C1/NPC1 protein, expressed by HEK293 , with N-His labeled tag. The total length of Niemann Pick C1/NPC1 Protein, Human (His-Flag) is 251 a.a., with molecular weight of ~38-55 kDa.

Niemann Pick C1 (NPC1) 蛋白作为细胞内胆固醇转运蛋白，与 NPC2 协同作用，促进胆固醇从内体/溶酶体室中流出。晚期内涵体/溶酶体中 LDL 释放的未酯化胆固醇被 NPC2 转移至 NPC1 N 末端结构域中的胆固醇结合袋。胆固醇与 NPC1 结合，其羟基埋在结合口袋中，与胆固醇相比，NPC1 对氧甾醇表现出更高的亲和力。除了胆固醇运输之外，NPC1 可能在神经胶质细胞的囊泡运输中发挥作用，这对于维持神经末梢的结构和功能完整性至关重要。此外，NPC1 通过与 SLC38A9 相互作用抑制胆固醇介导的 mTORC1 激活。在微生物感染的背景下，NPC1 作为埃博拉病毒的内体进入受体，突显了其在细胞过程中的多种作用及其在健康和疾病中的重要性。

Human

HEK293

N-6*His

O15118-1 (R372-F622)

4864  [NCBI]

RVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVF

Approximately 38-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.