Niemann Pick C2/NPC2 蛋白, Human (HEK293, His)

The Niemann Pick C2/NPC2 Protein is a crucial lysosomal protein that regulates cholesterol trafficking and lipid metabolism. It enables the movement of cholesterol from lysosomes to other parts of the cell. Studying the functions of NPC2 Protein is essential for understanding lipid disorders and developing treatments targeting disrupted cholesterol metabolism. Niemann Pick C2/NPC2 Protein, Human (HEK293, His) is the recombinant human-derived Niemann Pick C2/NPC2 protein, expressed by HEK293 , with C-His labeled tag.

Niemann Pick C2 (NPC2) 是一种重要的细胞内胆固醇转运蛋白，与 NPC1 协同作用，促进胆固醇从溶酶体中流出。当晚期内涵体/溶酶体腔内的 LDL 释放未酯化胆固醇时，NPC2 通过将胆固醇转移至位于 NPC1 N 末端结构域的胆固醇结合袋而发挥关键作用。值得注意的是，NPC2 在结合胆固醇方面表现出 1:1 的化学计量，并表现出结合各种甾醇的能力，包括弹甾醇、去莫甾醇以及豆甾醇和 β-谷甾醇等植物甾醇。此外，NPC2 可以结合并动员与膜相关的胆固醇。NPC2 的分泌形式通过刺激 ABCG5/ABCG8 介导的胆固醇转运进一步有助于调节胆汁胆固醇分泌。

Human

HEK293

C-His

P61916-1 (E20-L151)

10577  [NCBI]

EPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVSHL

21-24 kDa & 27-35 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.