Niemann Pick C2/NPC2 蛋白, Mouse (HEK293, His)

Niemann Pick C2/NPC2 Protein, a lysosomal protein, plays a critical role in cholesterol trafficking and lipid metabolism. It facilitates the transport of cholesterol from lysosomes to other cellular compartments. Understanding the functions of NPC2 Protein is vital for studying lipid disorders and developing therapeutic strategies targeting abnormal cholesterol metabolism. Niemann Pick C2/NPC2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Niemann Pick C2/NPC2 protein, expressed by HEK293 , with C-His labeled tag. The total length of Niemann Pick C2/NPC2 Protein, Mouse (HEK293, His) is 130 a.a., with molecular weight of ~15.9-19 kDa.

Niemann Pick C2 (NPC2) 充当细胞内胆固醇转运蛋白，与 NPC1 合作促进胆固醇从溶酶体区室流出。当晚期内体/溶酶体中的 LDL 释放未酯化胆固醇时，NPC2 通过将胆固醇转移到 NPC1 N 末端结构域中的胆固醇结合袋而发挥至关重要的作用。值得注意的是，NPC2 在结合胆固醇方面表现出 1:1 的化学计量，并表现出结合各种甾醇的能力，例如弹甾醇、去莫甾醇以及豆甾醇和 β-谷甾醇等植物甾醇。此外，NPC2的分泌形式有助于胆汁胆固醇分泌的调节，通过刺激ABCG5/ABCG8介导的胆固醇转运发挥其影响。

Mouse

HEK293

C-His

Q9Z0J0 (E20-S149)

67963  [NCBI]

EPLHFKDCGSKVGVIKEVNVSPCPTDPCQLHKGQSYSVNITFTSGTQSQNSTALVHGILEGIRVPFPIPEPDGCKSGINCPIQKDKVYSYLNKLPVKNEYPSIKLVVEWKLEDDKKNNLFCWEIPVQITS

Approximately 15.9-19 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.