NKG2D/CD314 蛋白, Human (sf9, His)

The NKG2D/CD314 protein serves as a key activating and costimulatory receptor in immune surveillance, binding to multiple stress-inducing ligands on tumor and virus-infected cells. It plays a dual role by stimulating NK cells and enhancing T cell activation in CD8(+) T cell-mediated adaptive responses. NKG2D/CD314 Protein, Human (sf9, His) is the recombinant human-derived NKG2D/CD314 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of NKG2D/CD314 Protein, Human (sf9, His) is 139 a.a., with molecular weight of ~22.6 kDa.

NKG2D/CD314 蛋白作为免疫监视所必需的激活和共刺激受体，与自体肿瘤细胞和病毒感染细胞上存在的多种细胞应激诱导配体结合。它在先天免疫反应中发挥双重作用，既刺激激活杀伤 (NK) 细胞，又在 CD8(+) T 细胞介导的适应性免疫反应中充当 T 细胞受体 (TCR) 的共刺激受体，从而增强 T 细胞激活。该受体促进穿孔素介导的配体表达肿瘤细胞的消除，其信号级联涉及钙内流，最终导致 TNF-α 表达。此外，NKG2D/CD314 参与 NK 细胞介导的骨髓移植排斥反应，并可能调节 NK 细胞的分化和存活。其配体结合能力延伸至 MHC I 类相关糖蛋白的各个亚家族，包括 MICA、MICB、RAET1E、RAET1G、RAET1L/ULBP6、ULBP1、ULBP2、ULBP3 (ULBP2>ULBP1>ULBP3) 和 ULBP4。该蛋白通过二硫键连接形成同二聚体，并与 HCST/DAP10 亚基形成异六聚体，这是 NK 细胞表面表达和细胞毒性诱导的关键相互作用。此外，它可以与 CD94 建立二硫键异二聚体，并与 CEACAM1 相互作用，招募 PTPN6 进行 VAV1 去磷酸化，同时不与 TYROBP 相互作用。

Immobilized human NKG2D-His (78-216) at 2 μg/mL (100 μL/well) can bind human ULBP1-hFc and the EC₅₀ is 8-50 ng/mL.

Human

Sf9 insect cells

N-His

P26718 (F78-V216)

22914  [NCBI]/100528032  [NCBI]

Approximately 22.6 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.