NLRP3 蛋白, Human (GST)

The NLRP3 protein acts as a sensor in the NLRP3 inflammasome, which is activated in response to membrane defects. It forms a complex with CASP1 and PYCARD/ASC, promotes the release of IL1B, IL18 and GSDMD, and induces pyroptosis. NLRP3 Protein, Human (GST) is the recombinant human-derived NLRP3 protein, expressed by E. coli , with N-GST labeled tag. The total length of NLRP3 Protein, Human (GST) is 574 a.a., with molecular weight of 93.20 kDa.

NLRP3 蛋白作为 NLRP3 炎症小体的传感器组件，协调炎症小体的激活，以响应损害膜完整性的各种刺激，导致炎症细胞因子 IL1B 和 IL18 的分泌以及细胞焦亡。在存在影响膜完整性的病原体或损伤相关信号的情况下，NLRP3 启动炎性体复合物的组装，该复合物包括 NLRP3、CASP1 和 PYCARD/ASC。这导致 caspase-1 前体的募集和激活，随后裂解并激活 IL1B、IL18 和 Gasdermin-D (GSDMD)，促进细胞因子释放和焦亡细胞死亡。激活刺激包括细胞外ATP、尼日利亚菌素、活性氧、晶体、β-淀粉样蛋白纤维和各种颗粒或纳米颗粒。有趣的是，NLRP3 还在调节辅助性 T 2 (Th2) 细胞的分化中发挥作用，有助于 Th2 细胞依赖性过程，例如哮喘和肿瘤生长。在 Th2 分化过程中，NLRP3 参与 IRF4 与 IL4 启动子的最佳结合以及 IRF4 依赖性 IL4 转录，突出了其除炎症体激活之外的多方面功能。

Human

E. coli

N-GST

Q96P20-2 (K131-V704)

114548  [NCBI]

KMKKDYRKKYRKYVRSRFQCIEDRNARLGESVSLNKRYTRLRLIKEHRSQQEREQELLAIGKTKTCESPVSPIKMELLFDPDDEHSEPVHTVVFQGAAGIGKTILARKMMLDWASGTLYQDRFDYLFYIHCREVSLVTQRSLGDLIMSCCPDPNPPIHKIVRKPSRILFLMDGFDELQGAFDEHIGPLCTDWQKAERGDILLSSLIRKKLLPEASLLITTRPVALEKLQHLLDHPRHVEILGFSEAKRKEYFFKYFSDEAQARAAFSLIQENEVLFTMCFIPLVCWIVCTGLKQQMESGKSLAQTSKTTTAVYVFFLSSLLQPRGGSQEHGLCAHLWGLCSLAADGIWNQKILFEESDLRNHGLQKADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEKEGRTNVPGSRLKLPSRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIEVKAKAKKLQIQPSQLELFYCLYEMQEEDFVQRAMDYFPKIEINLSTRMDHMVSSFCIENCHRVESLSLGFLHNMPKEEEEEEKEGRHLDMV

93.20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, 2 mM DTT, pH 8.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.