NLRP3 蛋白, Mouse (His-SUMO)

The NLRP3 protein acts as a sensor in the NLRP3 inflammasome, which is activated in response to membrane defects caused by pathogens or damage signals. It assembles the inflammasome complex, including NLRP3, CASP1, and PYCARD/ASC. NLRP3 Protein, Mouse (His-SUMO) is the recombinant mouse-derived NLRP3 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

NLRP3 蛋白充当 NLRP3 炎症小体的传感器组件，协调炎症小体激活以响应膜完整性缺陷。当遇到影响膜完整性的病原体或损伤相关信号时，NLRP3 会启动由 NLRP3、CASP1 和 PYCARD/ASC 组成的炎性聚合物复合物的组装。将 caspase-1 前体募集到 NLRP3 炎性体中，导致 caspase-1 激活，随后裂解并激活炎性细胞因子 IL1B 和 IL18，以及gasdermin-D (GSDMD)，导致细胞因子分泌和细胞焦亡。NLRP3 激活刺激涵盖一系列因素，包括细胞外 ATP、尼日利亚霉素、活性氧、晶体和各种环境颗粒。值得注意的是，几乎所有刺激都会诱导细胞内 K(+) 外流，从而导致膜扰动和 NLRP3 激活。激活的 NLRP3 被转运至微管组织中心 (MTOC)，并在与 NEK7 相互作用后重新定位至分散的跨高尔基体网络 (dTGN) 囊泡膜，形成活性炎症体复合物。此外，NLRP3 在 T 辅助细胞 2 (Th2) 细胞分化中发挥作用，通过调节参与这些途径的关键基因的转录来影响 Th2 细胞依赖性过程，例如哮喘和肿瘤生长。

Mouse

E. coli

N-SUMO;N-6*His

Q8R4B8-1 (M1-R153)

216799  [NCBI]

MTSVRCKLAQYLEDLEDVDLKKFKMHLEDYPPEKGCIPVPRGQMEKADHLDLATLMIDFNGEEKAWAMAVWIFAAINRRDLWEKAKKDQPEWNDTCTSHSSMVCQEDSLEEEWMGLLGYLSRISICKKKKDYCKMYRRHVRSRFYSIKDRNAR

Approximately 35 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCL, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.