NME1/NDPKA 蛋白, Human (His)

The NME1/NDPKA protein plays a crucial role in the synthesis of nucleoside triphosphates and exhibits multiple activities, including nucleoside diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease functions. NME1/NDPKA Protein, Human (His) is the recombinant human-derived NME1/NDPKA protein, expressed by E. coli , with N-6*His labeled tag.

NME1/NDPKA 蛋白在合成三磷酸核苷（ATP 除外）中起主要作用。它利用乒乓机制，通过磷酸化活性位点中间体将 ATP γ 磷酸转移到 NDP β 磷酸。该蛋白质表现出多种活性，包括核苷二磷酸激酶、丝氨酸/苏氨酸特异性蛋白激酶、香叶基/法呢基焦磷酸激酶、组氨酸蛋白激酶和3'-5'核酸外切酶。NME1 对于细胞增殖、分化、发育、信号转导、G 蛋白偶联受体内吞作用和基因表达至关重要。它对于神经发育至关重要，影响神经模式和细胞命运的决定。在 GZMA 介导的细胞死亡中，NME1 与 TREX1 合作，在一条 DNA 链上产生切口并增强 DNA 损伤，以防止快速修复和重新退火。

Human

E. coli

N-6*His

P15531-1 (M1-E152)

4830  [NCBI]

MANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE

Approximately 20.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 1 mM DTT, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.