NOTCH1 蛋白, Human (HEK293, Fc)

NOTCH1 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is critical for cell fate decisions. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer. NOTCH1 Protein, Human (HEK293, Fc) is the recombinant human-derived NOTCH1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of NOTCH1 Protein, Human (HEK293, Fc) is 508 a.a., with molecular weight of ~79.7 kDa.

NOTCH1 蛋白作为膜结合配体的受体，包括 Jagged-1 (JAG1)、Jagged-2 (JAG2) 和 Delta-1 (DLL1)，在细胞命运决定中发挥关键的调节功能。配体激活后，释放的 Notch 胞内结构域 (NICD) 与 RBPJ/RBPSUH 形成转录激活剂复合物，协调分裂位点增强子内基因的激活。NOTCH1 显着影响细胞分化、增殖和凋亡程序。除了在血管生成中负向调节内皮细胞增殖和迁移的作用外，NOTCH1 还参与胸腺成熟、滤泡分化和滤泡内的细胞命运选择。此外，它作为神经元 DNER 的受体，在小脑发育中发挥着至关重要的作用，有助于伯格曼胶质细胞的分化。NOTCH1 还在植入后发育、中胚层发育、体节形成和神经发生中发挥作用。它与多种蛋白质相互作用，包括 DNER、DTX1、DTX2、RBPJ/RBPSUH、MAML1、MAML2、MAML3、SNW1、AAK1、FBXW7、SGK1、HIF1AN、SNAI1、MDM2A、BCL6、THBS4、CCN3、DLL4、ZMIZ1、MEGF10、PRAG1 、PSEN1 和 ZFP64，形成一个调节不同细胞过程和调节途径的复杂网络。

Immobilized Recombinant DLL4 Protein at 5 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human NOTCH1 Protein. The ED₅₀ for this effect is 0.1495 μg/mL.

Human

HEK293

C-hFc

P46531/NP_060087.3 (A19-Q526)

4851  [NCBI]

ARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQ

Approximately 79.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.