Notch3 蛋白, Human (HEK293, Fc)

Notch 3 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and plays a crucial regulatory role in cell fate determination. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes within the enhancer of the cleavage site. Notch3 Protein, Human (HEK293, Fc) is the recombinant human-derived Notch3 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Notch3 Protein, Human (HEK293, Fc) is 428 a.a., with molecular weight of 75-83 kDa.

Notch 3 蛋白作为膜结合配体（包括 Jagged1、Jagged2 和 Delta1）的受体，在调节细胞命运决定中发挥着关键作用。配体激活后，释放的缺口胞内结构域 (NICD) 与 RBPJ/RBPSUH 形成转录激活剂复合物，启动分裂位点增强子内基因的激活。这种多方面的蛋白质影响细胞分化、增殖和凋亡程序。在结构上，它以异二聚体形式存在，由 C 端片段 (N(TM)) 和 N 端片段 (N(EC)) 组成，可能通过二硫键连接。Notch 3 与转录共激活因子 MAML1、MAML2 和 MAML3 相互作用，调节下游转录过程。它还与 PSMA1 和 HIF1AN 结合，促进多种细胞功能和调节途径。

Human

HEK293

C-hFc

Q9UM47 (A40-E467)

4854  [NCBI]

75-83 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.