NOV/CCN3 蛋白, Canine (HEK293, His)

NOV/CCN3 Protein belongs to an emerging family of growth regulators referred under the CCN acronym. NOV/CCN3 Protein enables heparin and binding. NOV/CCN3 Protein is involved in cell adhesion and signal transduction. NOV/CCN3 Protein, Canine (HEK293, His) is the recombinant canine-derived NOV/CCN3 protein, expressed by HEK293 , with C-His labeled tag. The total length of NOV/CCN3 Protein, Canine (HEK293, His) is 325 a.a., with molecular weight of 42-50 KDa.

NOV/CCN3 蛋白属于一个新兴的生长调节因子家族，简称为 CCN。CCN 家族目前包括 6 个成员，它们具有细胞生长的正调节因子和负调节因子的特性，共享一个共同的多模块组织。NOV/CCN3 蛋白使肝素和结合成为可能。NOV/CCN3 蛋白参与细胞黏附和信号转导^[1][2][3]。

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion, rhNOV, immobilized at 10 µg/mL, will induce 62.85% adhesion on Balbc/3T3 cells (100 µL/well at 3 x 10⁴ cells/mL).

Canine

HEK293

C-6*His

J9P4N7 (Q29-M353)

475083  [NCBI]

QRCPTQCPARCAPTPPACAPGVRAVLDDCSCCLVCARQRGESCSPLQPCEESRGLFCDRRADPSAGGGICMAVEGDNCVFDGVIYQSGETFQPSCKYQCACRDGQIGCVPRCGEDLLLPQPDCPAPRKVEVPGECCEKWICDSNEKGELGGLALPAYRTEATLGVAVSDSGINCIEQTTEWSACSKSCGMGFSTRVTNRNPQCEMVKQTRLCMVRPCEQEHQQPADKKGKKCLRTTKSLKAIHLQFKNCTSLHTYKPRFCGVCSDGRCCTPHNTKTIQVEFQCSPGQIIKKPVMVIGTCTCHSNCPHNREAFLQELKPNTSRGEM

Approximately 42-50 due to the glycosylation

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.