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  3. NOV/CCN3 蛋白, Rhesus Macaque (sf9, His)

NOV/CCN3 蛋白, Rhesus Macaque (sf9, His)

目录号: HY-P77454
产品使用指南

NOV/CCN3 蛋白参与多种生物过程,包括迁移、分化、生存和炎症。它通过结合整合素或膜受体(例如 NOTCH1)发挥作用。NOV/CCN3 蛋白, Rhesus Macaque (sf9, His) 是重组的 NOV/CCN3 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。NOV/CCN3 蛋白, Rhesus Macaque (sf9, His) 全长 326 个氨基酸,分子量约为 ~37.2 KDa。

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描述

NOV/CCN3 proteins are involved in a variety of biological processes, including migration, differentiation, survival, and inflammation. It acts by binding to integrins or membrane receptors such as NOTCH1. NOV/CCN3 Protein, Rhesus Macaque (sf9, His) is the recombinant Rhesus Macaque-derived NOV/CCN3 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of NOV/CCN3 Protein, Rhesus Macaque (sf9, His) is 326 a.a., with molecular weight of ~37.2 KDa.

研究背景

NOV/CCN3蛋白参与多种生物过程,包括迁移、分化、存活和炎症。它通过结合整合素或膜受体(例如 NOTCH1)发挥作用。它是造血干细胞和祖细胞的重要调节剂,并通过激活 Notch 信号通路抑制肌原性分化。它还抑制血管平滑肌细胞增殖,支持内皮细胞粘附、迁移和存活,促进血管生成。在皮肤伤口愈合中,它充当整合素受体配体,支持成纤维细胞粘附和趋化性。它参与骨再生并增强关节软骨细胞表型,同时抑制软骨内骨化。它损害胰腺 β 细胞功能,并作为内皮促炎症激活的负调节因子发挥作用,减少单核细胞粘附并抑制 NF-kappaB 信号通路。此外,它还有助于控制和协调动脉粥样硬化中的炎症过程,并通过调节 MMP9、MMP2 和 CCL2 的表达来减轻炎症疼痛。

种属

Rhesus Macaque

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

F6WCM6 (T32-M357)

基因 ID
蛋白结构
N-term
CCN3 (T32-M357)
Accession # F6WCM6
His
C-term
同用名
Nephroblastoma Overexpressed Gene Protein; IBP-9; CCN3; IGFBP9; NOVH
分子量

Approximately 37.2 kDa.

纯度

Greater than 80% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NOV/CCN3 Protein, Rhesus Macaque (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NOV/CCN3 Protein, Rhesus Macaque (sf9, His)
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HY-P77454
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