NPPB 蛋白, Human (His)

NPPB Protein, a member of the natriuretic peptide family, functions as a key regulator of cardiovascular homeostasis. As a hormone, NPPB is primarily produced and released by cardiac ventricular myocytes in response to increased pressure and volume. Upon secretion, it acts on target tissues, including the heart and blood vessels, to induce vasodilation, natriuresis, and diuresis, collectively contributing to the maintenance of blood pressure and fluid balance. NPPB's pleiotropic effects underscore its crucial role in cardiovascular physiology and its potential therapeutic implications in managing cardiovascular disorders. NPPB Protein, Human (His) is the recombinant human-derived NPPB protein, expressed by E. coli, with N-6*His labeled tag. The total length of NPPB Protein, Human (His) is 108 a.a., with molecular weight of ~15 kDa.

TRIM5 蛋白作为衣壳特异性限制因子，通过在病毒生命周期早期（特别是在病毒进入后但逆转录之前）阻断病毒复制来阻止非宿主适应逆转录病毒的感染。除了作为衣壳特异性限制因子的作用外，TRIM5 还充当模式识别受体，激活先天免疫信号以响应逆转录病毒衣壳晶格。与病毒衣壳结合后，TRIM5 触发其 E3 泛素连接酶活性，与 UBE2V1-UBE2N 复合物协作生成“Lys-63”连接的多聚泛素链。这种泛素化过程导致 MAP3K7/TAK1 复合物的自身磷酸化，从而诱导和表达 NF-kappa-B 和 MAPK 反应性炎症基因，最终在受感染细胞中启动先天免疫反应。TRIM5 的限制能力扩展到各种逆转录病毒，包括 N-tropic 鼠白血病病毒 (N-MLV)、马传染性贫血病毒 (EIAV)、猕猴猿免疫缺陷病毒 (SIVmac)、猫免疫缺陷病毒 (FIV) 和牛免疫缺陷病毒 (FIV)。BIV）。此外，TRIM5 通过激活自噬调节因子 BECN1，导致其与抑制剂 BCL2 和 TAB2 解离，在调节自噬中发挥着至关重要的作用。此外，TRIM5 作为选择性自噬受体，识别并靶向 HIV-1 衣壳蛋白 p24 进行自噬降解。

Human

E. coli

N-6*His

P16860 (H27-H134)

4879  [NCBI]

HPLGSPGSASDLETSGLQEQRNHLQGKLSELQVEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPRSPKMVQGSGCFGRKMDRISSSSGLGCKVLRRH

Approximately 15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.