NPPB 蛋白, Human (76a.a, Flag-His)

NPPB proteins are members of the natriuretic peptide family and are key regulators of cardiovascular homeostasis. As a hormone, NPPB is produced and released primarily by ventricular myocytes in response to increases in pressure and volume. NPPB Protein, Human (76a.a, Flag-His) is the recombinant human-derived NPPB protein, expressed by E. coli , with N-6*His, N-Flag labeled tag. The total length of NPPB Protein, Human (76a.a, Flag-His) is 76 a.a., with molecular weight of ~14.0 kDa.

TRIM5 蛋白作为衣壳特异性限制因子，通过在病毒生命周期早期（特别是在病毒进入后但逆转录之前）阻断病毒复制来阻止非宿主适应逆转录病毒的感染。除了作为衣壳特异性限制因子的作用外，TRIM5 还充当模式识别受体，激活先天免疫信号以响应逆转录病毒衣壳晶格。与病毒衣壳结合后，TRIM5 触发其 E3 泛素连接酶活性，与 UBE2V1-UBE2N 复合物协作生成“Lys-63”连接的多聚泛素链。这种泛素化过程导致 MAP3K7/TAK1 复合物的自身磷酸化，从而诱导和表达 NF-kappa-B 和 MAPK 反应性炎症基因，最终在受感染细胞中启动先天免疫反应。TRIM5 的限制能力扩展到各种逆转录病毒，包括 N-tropic 鼠白血病病毒 (N-MLV)、马传染性贫血病毒 (EIAV)、猕猴猿免疫缺陷病毒 (SIVmac)、猫免疫缺陷病毒 (FIV) 和牛免疫缺陷病毒 (FIV)。BIV）。此外，TRIM5 通过激活自噬调节因子 BECN1，导致其与抑制剂 BCL2 和 TAB2 解离，在调节自噬中发挥着至关重要的作用。此外，TRIM5 作为选择性自噬受体，识别并靶向 HIV-1 衣壳蛋白 p24 进行自噬降解。

Human

E. coli

N-6*His;N-Flag

P16860 (H27-R102)

4879  [NCBI]

HPLGSPGSASDLETSGLQEQRNHLQGKLSELQVEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPR

Approximately 14.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.