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  4. NRN1L 蛋白, Human (HEK293, His)

NRN1L 蛋白, Human (HEK293, His)

目录号: HY-P71172
产品使用指南

NRN1L 蛋白由细胞外的 nrn1l 基因编码，可增强神经突生长和神经元存活。NRN1L 蛋白以 GPI 锚定膜结合形式和分泌形式存在。该活性相关配体作为同二聚体或异二聚体发挥作用。NRN1L 蛋白属于神经突蛋白 (Nrn1) 家族。NRN1 参与神经发育、突触可塑性和突触成熟。NRN1L 蛋白, Human (HEK293, His) 是重组的 NRN1L 蛋白，由 HEK293 表达，带有 C-6*His 标签。NRN1L 蛋白, Human (HEK293, His) 全长 104 个氨基酸，分子量分别为 14 & 16 kDa。

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描述

NRN1L Protein is encoded by nrn1l gene in extracellular and enhances both neurite growth and neuronal survival. NRN1L protein is found both as a GPI anchored membrane-bound form and as a secreted form. This activity-related ligand functions as a homodimer or heterodimer. NRN1L Protein belongs to the neuritin (Nrn1) family. NRN1 is involved in neural development, synaptic plasticity, and synapse maturation[1][2]. NRN1L Protein, Human (HEK293, His) is the recombinant human-derived NRN1L protein, expressed by HEK293, with C-6*His labeled tag. The total length of NRN1L Protein, Human (HEK293, His) is 104 a.a., with molecular weight of 14 & 16 kDa, respectively.
研究背景

Neuritin (Nrn1) 是一种糖磷脂酰肌醇连接蛋白。Nrn1 在包括神经系统在内的各种人体组织中表达，特别是在细胞膜的脂筏中。Nrn1 促进视觉皮层神经元的神经突生长、树突生长、神经元迁移和突触成熟；它还调节突触可塑性、周围神经元凋亡和脊髓轴突再生，并促进脑缺血后的恢复[2]。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

Q496H8 (A36-A139)
基因 ID
蛋白结构
N-term
NRN1L (A36-A139)
Accession # Q496H8
6*His
C-term
同用名
Neuritin-like protein; NRN1L; UNQ2446/PRO5725
氨基酸序列

AAGPNRCDTIYQGFAECLIRLGDSMGRGGELETICRSWNDFHACASQVLSGCPEEAAAVWESLQQEARQAPRPNNLHTLCGAPVHVRERGTGSETNQETLRATA

分子量

14&16 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

NRN1L Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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