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  3. SARS-CoV-2 NSP3 蛋白 (A1-T183)

SARS-CoV-2 NSP3 蛋白 (A1-T183)

目录号: HY-P703535
产品使用指南

多功能 PL-PRO 蛋白在病毒 RNA 转录和复制中发挥着关键作用,为多蛋白裂解提供必要的蛋白酶。它通过与 40S 亚基的开放头构象结合来战略性地阻断宿主翻译,阻止核糖体 mRNA 进入隧道。NSP3 蛋白, SARS-CoV-2 (A1-T183) 是重组的 NSP3 蛋白,由 E. coli 表达,带有 tag Free 标签。NSP3 蛋白, SARS-CoV-2 (A1-T183)

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描述

The multifunctional PL-PRO protein plays a critical role in viral RNA transcription and replication, providing the necessary proteases for multiprotein cleavage. It strategically blocks host translation by binding to the open head conformation of the 40S subunit, impeding ribosomal mRNA entry into the tunnel. NSP3 Protein, SARS-CoV-2 (A1-T183) is the recombinant virus-derived NSP3, expressed by E. coli , with tag Free labeled tag. ,

研究背景

PL-PRO 蛋白在病毒 RNA 的复杂转录和复制过程中发挥着多功能关键作用,承载着负责多蛋白裂解的关键蛋白酶。从功能上讲,它通过与 40S 亚基的开放头构象结合来阻碍宿主翻译,并且其 C 末端错综复杂地结合并阻碍核糖体 mRNA 进入通道。这种干扰在抑制先天免疫或干扰素引发的抗病毒反应中发挥着关键作用。由 PL-PRO 精心策划的 nsp1-40S 核糖体复合物的协同形成,在宿主 mRNA 的 5'UTR 附近诱导核酸内切切割,标记它们进行降解。值得注意的是,病毒 mRNA 对 nsp1 介导的翻译抑制表现出更强的抵抗力,这归因于其独特的 5' 端前导序列。PL-PRO 的多方面功能强调了其在操纵宿主细胞过程、促进病毒复制和逃避宿主防御方面不可或缺的作用。

种属

Virus

表达系统

E. coli

标签

Tag Free

蛋白编号

P0DTC1 (A1-T183)

基因 ID

同用名
Replicase polyprotein 1a; ORF1a polyprotein; Severe acute respiratory syndrome coronavirus 2; 2019-nCoV; pp1a; Endonuclease; Hydrolase; Methyltransferase; Nuclease; Protease; RNA-binding; Thiol protease; Transferase
分子量

Approximately 20.7 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SARS-CoV-2 NSP3 Protein (A1-T183) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SARS-CoV-2 NSP3 Protein (A1-T183)
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HY-P703535
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