Nucleocapsid 蛋白 CTD domain, Virus (His)

Nucleocapsid Protein CTD domain, Virus (His) is the C-terminal dimerisation domain (CTD) of nucleocapsid protein in coronaviruses. Nucleocapsid Protein CTD domain has a stronger nucleic acid-binding activity than the NTD, which could be a nucleic acid-binding site^[1] [2].

冠状病毒是具有正义RNA基因组的有包膜病毒，并具有螺旋对称的核衣壳。在病毒感染的原代细胞和用表达 N 蛋白的质粒转染的细胞中，核衣壳蛋白定位于细胞质和核仁。核衣壳蛋白是一种碱性蛋白，在冠状病毒中含量丰富，对核酸（包括 ssRNA、ssDNA 和 dsDNA）具有非特异性结合活性^[1]。
SARS-CoV N 蛋白包含两个不同的RNA结合结构域（N端结构域[NTD]和CTD，它们通过结构不良的连接区域连接。跨越残基248至365的CTD显示出比NTD和之间的碱性区域更强的核酸结合活性CTD 的残基 248 至 280 形成带正电的凹槽，代表可能的 RNA 结合区域。此外，冠状病毒 NP-CTD 的晶体结构提供了结构线索，说明冠状病毒核衣壳的构建模块如何通过 NP 二聚体形成^[2] 。

Virus

E. coli

N-6*His

P0DTC9/QHD43423.2 (A119-Q239)

43740575  [NCBI]

N/A

Approximately 16.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of PBS, 2 M Urea, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Sander van Boheemen, et al. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. mBio. 2012 Nov 20;3(6):e00473-12.  [Content Brief]

  [2]. Chun-Yuan Chen, et al. Structure of the SARS coronavirus nucleocapsid protein RNA-binding dimerization domain suggests a mechanism for helical packaging of viral RNA. J Mol Biol. 2007 May 11;368(4):1075-86.  [Content Brief]