Nucleophosmin/Npm1 蛋白, Human (150a.a, His)

The nucleophosmin/Npm1 protein plays critical roles in ribosome biogenesis, centrosome duplication, protein chaperones, histone assembly, and cell proliferation. Npm1 is a key player in the cellular orchestra that promotes ribosome nuclear export, associates with nucleolar ribonucleoprotein structures, and interacts with single-stranded nucleic acids. Nucleophosmin/Npm1 Protein, Human (150a.a, His) is the recombinant human-derived Nucleophosmin/Npm1 protein, expressed by E. coli , with N-His labeled tag.

核磷蛋白/Npm1 蛋白处于多种细胞过程的十字路口，在核糖体生物合成、中心体复制、蛋白质伴侣、组蛋白组装和细胞增殖的复杂调节中发挥着关键作用。这种多面蛋白质成为细胞乐团中的关键参与者，与核糖体结合以促进其核输出，与核仁核糖核蛋白结构结合，并与单链核酸相互作用。Npm1 作为伴侣蛋白，与核心组蛋白 H3、H2B 和 H4 协同作用，刺激 APEX1 对双链 DNA 的核酸内切酶活性，同时抑制其对单链 RNA 的活性。除了参与核仁过程之外，Npm1 还调节中心体和中心粒复制、抵抗细胞凋亡，并与 MYC 一起调节 MYC 靶基因的转录活性。复杂的交互网络涉及 NSUN2、SENP3、RPS10、NEK2、ROCK2、BRCA2、RPGR、CENPW、EIF2AK2/PKR、CEBPA、DDX31、NOP53、LRRC34、RRP1B、NPM3、ALKBH2 和 TTF1 等合作伙伴，强调了复杂性和Npm1 在细胞功能中的多功能性。

Human

E. coli

N-His

P06748-1 (M9-L158)

4869  [NCBI]

MSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKL

Approximately 19 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 20% Acetonitrile, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.