Nucleoside phosphorylase/PNP 蛋白, Human (His)

Nucleoside phosphorylase/PNP proteins play a vital role in cellular processes by catalyzing the phosphate breakdown of β-(deoxy)ribonucleosides, preferably 6-oxopurine nucleosides, such as inosine and guanosine. This enzymatic activity results in the formation of free purine bases and pentose 1-phosphate, highlighting the importance of this protein in regulating nucleoside degradation. Nucleoside phosphorylase/PNP Protein, Human (His) is the recombinant human-derived Nucleoside phosphorylase/PNP protein, expressed by E. coli , with C-10*His labeled tag. The total length of Nucleoside phosphorylase/PNP Protein, Human (His) is 289 a.a., with molecular weight of ~33.5 kDa.

核苷磷酸化酶/PNP 蛋白通过催化 β-（脱氧）核糖核苷分子中 N-糖苷键的磷酸分解，在细胞过程中发挥着至关重要的作用。如相关文献所述，这种酶活性导致相应的游离嘌呤碱基和 1-磷酸戊糖的形成。值得注意的是，该蛋白质表现出对 6-氧代嘌呤核苷的偏好，例如肌苷和鸟苷。核苷磷酸化酶/PNP 的底物特异性强调了其在调节核苷降解中的重要性，深入了解其在维持细胞嘌呤稳态和促进核苷酸代谢整体动态方面的作用。

Measured by the phosphorolysis of 7-methyl-6-thioguanosine. The specific activity is 4672.43 pmol/min/µg, as measured under the described conditions.

Human

E. coli

C-10*His

P00491 (M1-S289)

4860  [NCBI]

MENGYTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASIPLPDKAS

Approximately 33.5 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of PBS, 25% glycerol, pH 7.5 or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.