NUDT5 蛋白, Human (His)

The NUDT5 protein acts as an ADP-glycopyrophosphatase or synthesizes ATP. NUDT5 Protein, Human (His) is the recombinant human-derived NUDT5 protein, expressed by E. coli , with N-His labeled tag. The total length of NUDT5 Protein, Human (His) is 218 a.a., with molecular weight of 30-35 KDa.

NUDT5 是一种多功能酶，具有双重功能：在不存在二磷酸盐的情况下充当 ADP-糖焦磷酸酶，并在存在二磷酸盐的情况下催化 ATP 合成。在没有二磷酸盐的情况下，NUDT5 对各种修饰的核苷二磷酸盐表现出水解活性，包括 ADP-核糖、ADP-甘露糖、ADP-葡萄糖、8-oxo-GDP 和 8-oxo-dGDP。此外，它还可以水解其他低活性的核苷酸糖。当 Thr-45 去磷酸化时，NUDT5 通过将 ADP-核糖转化为 ATP 和核糖 5-磷酸，促进细胞核中 ATP 的合成。这种核 ATP 的产生对于消耗能量的染色质重塑事件至关重要。尽管具有多种酶活性，NUDT5 在 U8 snoRNA 脱帽活性中并不发挥作用，尽管它表现出与 U8 snoRNA 的结合亲和力。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

Q9UKK9 (E2-F219)

11164  [NCBI]

ESQEPTESSQNGKQYIISEELISEGKWVKLEKTTYMDPTGKTRTWESVKRTTRKEQTADGVAVIPVLQRTLHYECIVLVKQFRPPMGGYCIEFPAGLIDDGETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCTIHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAKPFEVPFLKF

30-35 kDa.

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4(Normally 8% trehalose is added as protectant before lyophilization) or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.