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  3. OBCAM/OPCML 蛋白, Rat (HEK293, Fc)

OBCAM/OPCML 蛋白, Rat (HEK293, Fc)

目录号: HY-P77115
COA 产品使用指南

OBCAM/OPCML 蛋白在酸性脂质环境中结合阿片类药物,影响细胞通讯并促进细胞接触,可能影响各种生物过程。OBCAM/OPCML 蛋白, Rat (HEK293, Fc) 是重组的 OBCAM/OPCML 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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规格 价格 是否有货 数量
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5 μg ¥470 In-stock
10 μg ¥800 In-stock
50 μg ¥2200 In-stock
100 μg ¥3750 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

OBCAM/OPCML Protein binds opioids in acidic lipid environments, influencing cellular communication and facilitating cell contact, potentially impacting various biological processes. OBCAM/OPCML Protein, Rat (HEK293, Fc) is the recombinant rat-derived OBCAM/OPCML protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

OBCAM/OPCML 蛋白通过在酸性脂质存在下与阿片类药物结合而发挥作用,可能在细胞通讯中发挥作用。这种蛋白质参与促进细胞接触,并可能对各种生物过程产生影响。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Rat OBCAM at 1 μg/mL (100 μL/well) can bind biotinylated human LSAMP. The ED50 for this effect is 0.7336 μg/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Rat OBCAM at 1 μg/mL (100 μL/well) can bind biotinylated human LSAMP. The ED50 for this effect is 0.7336 μg/mL.
种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

P32736-1 (G28-V321)

基因 ID
蛋白结构
N-term
OPCML (G28-V321)
Accession # P32736-1
hFc
C-term
同用名
Opioid-binding protein/cell adhesion molecule; OBCAM; OPCML; IGLON1
氨基酸序列

GVPVRSGDATFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYAGNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITVNEISSVTLLCLAIGRPEPTVTWRHLSVKEGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVKITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEDTRLATGLDGVRIENKGRISTLTFFNVSEKDYGNYTCVATNKLGNTNASITLYGPGAVIDGV

分子量

Approximately 90 kDa.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

OBCAM/OPCML Protein, Rat (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
OBCAM/OPCML Protein, Rat (HEK293, Fc)
目录号:
HY-P77115
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