OSM 蛋白, Human (227a.a, His)

OSM protein is a multifunctional growth regulator with dual functions of inhibiting tumor cell lines and stimulating the proliferation of AIDS-KS cells. It coordinates the production of cytokines, including IL-6, G-CSF, and GM-CSF, and binds to type I and type II OSM receptors, forming heterodimers with LIFR/IL6ST and OSMR/IL6ST, respectively. OSM Protein, Human (227a.a, His) is the recombinant human-derived OSM protein, expressed by E. coli , with N-6*His labeled tag. The total length of OSM Protein, Human (227a.a, His) is 227 a.a., with molecular weight of ~27 kDa.

GMP OSM 蛋白是一种多功能生长调节剂，对多种肿瘤细胞系的增殖具有双重抑制作用，对 AIDS-KS 细胞增殖具有刺激作用。值得注意的是，OSM 协调细胞因子产生的调节，包括内皮细胞的 IL-6、G-CSF 和 GM-CSF。这种多方面的生长调节剂既与 I 型 OSM 受体结合，形成由 LIFR 和 IL6ST 组成的异二聚体，又与 II 型 OSM 受体结合，形成由 OSMR 和 IL6ST 组成的异二聚体。除了其抗增殖和增殖作用之外，OSM 在胎儿肝细胞的成熟中发挥着至关重要的作用，对肝脏发育和再生做出了重大贡献。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.2968 ng/mL, corresponding to a specific activity is 3.369×10⁶ U/mg.

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  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.2968 ng/mL, corresponding to a specific activity is 3.369×10⁶ U/mg.

Human

E. coli

N-6*His

P13725 (A26-R252)

5008  [NCBI]

AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRRHSPHQALRKGVRRTRPSRKGKRLMTRGQLPR

Approximately 27 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.