OSM 蛋白, Human (His)

OSM protein is a multifunctional growth regulator with dual functions of inhibiting tumor cell lines and stimulating the proliferation of AIDS-KS cells. It coordinates the production of cytokines, including IL-6, G-CSF, and GM-CSF, and binds to type I and type II OSM receptors, forming heterodimers with LIFR/IL6ST and OSMR/IL6ST, respectively. OSM Protein, Human (His) is the recombinant human-derived OSM protein, expressed by E. coli , with N-6*His labeled tag. The total length of OSM Protein, Human (His) is 196 a.a., with molecular weight of ~24 kDa.

GMP OSM 蛋白是一种多功能生长调节剂，对多种肿瘤细胞系的增殖具有双重抑制作用，对 AIDS-KS 细胞增殖具有刺激作用。值得注意的是，OSM 协调细胞因子产生的调节，包括内皮细胞的 IL-6、G-CSF 和 GM-CSF。这种多方面的生长调节剂既与 I 型 OSM 受体结合，形成由 LIFR 和 IL6ST 组成的异二聚体，又与 II 型 OSM 受体结合，形成由 OSMR 和 IL6ST 组成的异二聚体。除了其抗增殖和增殖作用之外，OSM 在胎儿肝细胞的成熟中发挥着至关重要的作用，对肝脏发育和再生做出了重大贡献。

The dose-dependent stimulation of TF 1 human erythroleukemic cells has an ED50 value of 0.2-1 ng/mL.

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  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.2921 ng/mL, corresponding to a specific activity is 3.423×10⁶ units/mg.

Human

E. coli

N-6*His

P13725 (A26-R221)

5008  [NCBI]

AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRR

Approximately 24 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 200 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.