Osteocrin 蛋白, Human (His)

Osteocrin protein regulates dendritic growth in the developing cerebral cortex by inhibiting branching. It is induced in the brain upon membrane depolarization and binds to the natriuretic peptide receptor NPR3/NPR-C, preventing the interaction with natriuretic peptides and increasing cproduction. Osteocrin also interacts with NPR3, further modulating dendritic growth regulation. Osteocrin Protein, Human (His) is the recombinant human-derived Osteocrin protein, expressed by E. coli , with N-6*His labeled tag. The total length of Osteocrin Protein, Human (His) is 106 a.a., with molecular weight of ~12.0 kDa.

骨分泌蛋白是发育中的大脑皮层树突生长的重要调节因子，对感官体验做出反应。它是在膜去极化时在大脑中诱导的，并抑制发育中皮层神经元的树突分支。该蛋白可能通过与利钠肽受体 NPR3/NPR-C 结合来实现其作用，从而阻止 NPR3/NPR-C 与利钠肽之间的相互作用，最终导致 c 产量增加。此外，骨分泌蛋白与 NPR3 相互作用，进一步调节其在树突生长调节中的活性。

Human

E. coli

N-6*His

P61366 (V28-G133)

344901  [NCBI]

VDVTTTEAFDSGVIDVQSTPTVREEKSATDLTAKLLLLDELVSLENDVIETKKKRSFSGFGSPLDRLSAGSVDHKGKQRKVVDHPKRRFGIPMDRIGRNRLSNSRG

Approximately 12.0-13.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.