Osteopontin/OPN 蛋白, Human (HEK293, His)

Osteopontin/OPN Protein, a key non-collagenous bone protein, strongly binds to hydroxyapatite, contributing to the mineralized matrix. Its significant role in cell-matrix interactions is evident. Acting as a cytokine, OPN promotes interferon-gamma and interleukin-12 production while suppressing interleukin-10, crucial for type I immunity. Osteopontin/OPN Protein, Human (HEK293, His) is the recombinant human-derived Osteopontin/OPN protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Osteopontin/OPN Protein, Human (HEK293, His) is 298 a.a., with molecular weight of 50-65 kDa.

骨桥蛋白 (OPN) 是一种重要的非胶原骨蛋白，与羟基磷灰石具有很强的结合亲和力，使其成为矿化基质的组成部分。它可能在细胞-基质相互作用中发挥着至关重要的作用。此外，OPN 作为细胞因子，促进干扰素-γ 和白细胞介素 12 的产生，同时抑制白细胞介素 10 的产生。这些功能对于促进 I 型免疫的途径至关重要。

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED₅₀ for this effect is <0.1817 μg/mL, corresponding to a specific activity is >5503.58 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10⁵ cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.1486 μg/mL, corresponding to a specific activity is 6729.475 units/mg.

Human

HEK293

C-6*His

P10451-1 (I17-N314)

6696  [NCBI]

IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN

Approximately 50-69 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.