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Outer membrane 蛋白 A/OmpA 蛋白, E.coli (His-SUMO)

目录号: HY-P71493
COA 产品使用指南

外膜蛋白 A (OmpA) 确保细菌细胞壁的结构完整性,并与 TolR 配合稳定肽聚糖层。作为孔蛋白,OmpA 控制小溶质的渗透性。Outer membrane 蛋白 A/OmpA 蛋白, E.coli (His-SUMO) 是重组的 外膜蛋白 A/OmpA 蛋白,由 E. coli 表达,带有 N-SUMO, N-6*His 标签。Outer membrane 蛋白 A/OmpA 蛋白, E.coli (His-SUMO) 全长 183 个氨基酸,分子量约为 ~35.6 kDa。

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描述

Outer membrane protein A (OmpA) ensures the structural integrity of the bacterial cell wall and cooperates with TolR to stabilize the peptidoglycan layer. As a porin, OmpA controls the permeability of small solutes. Outer membrane protein A/OmpA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Outer membrane protein A/OmpA protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Outer membrane protein A/OmpA Protein, E.coli (His-SUMO) is 183 a.a., with molecular weight of ~35.6 kDa.

研究背景

外膜蛋白 A (OmpA) 在细菌细胞壁的结构完整性中发挥着至关重要的作用,与 TolR 协同作用以维持肽聚糖层在周质中的位置。作为孔蛋白,OmpA 表现出低渗透性,有助于小溶质逐渐渗透其结构。OmpA 内的内门进一步调节溶质的通过,影响分子的选择性运输。此外,OmpA 的重要性还在于它参与与 F 型质粒的接合,它可能作为受体细胞上的重要交配受体,有助于遗传物质的水平转移。

种属

E.coli

表达系统

E. coli

标签

N-SUMO;N-6*His

蛋白编号

P0A911 (W164-A346)

基因 ID

917123  [NCBI]

蛋白结构
N-term
6*His-SUMO
OmpA (W164-A346)
Accession # P0A911
C-term
同用名
ompA; Z1307; ECs1041; Outer membrane protein A; Outer membrane porin A
氨基酸序列

WTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGIKDVVTQPQA

分子量

Approximately 35.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Outer membrane protein A/OmpA Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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