OX40 Ligand/TNFSF4 蛋白, Mouse (HEK293, rFc)

OX40 Ligand (TNFSF4) is a type II glycoprotein with a cytoplasmic tail of 23 aa and an extracellular domain of 133 aa^[1]. OX40 Ligand is a ligand for TNFRSF4 (CD134), belongs to tumor necrosis factor (TNF) family. OX40 Ligand can activate OX40 and thereby functioning as a T cell co-stimulatory molecule. The OX40-OX40 Ligand interaction promotes effector T-cell survival and effectively induces memory T-cell generation, as well as enhances the helper function of Tfh for B cells, and also promotes the differentiation and maturation of DCs^[1][2]. OX40 Ligand/TNFSF4 Protein, Mouse (HEK293, rFc) is a recombinant mouse OX40 Ligand (Q49-L198) with N-terminal rFc tag, which is produced in HEK293.

OX40 配体 (TNFSF4) 是一种 II 型糖蛋白，具有 23 个氨基酸的胞质尾部和 133 个氨基酸的胞外结构域^[1]。OX40 配体在抗原呈递细胞上表达，例如 B 细胞、树突状细胞 (DC) 和巨噬细胞以及气道平滑肌细胞^[3]。OX40 Ligand是TNFRSF4 (CD134)的配体，属于肿瘤坏死因子(TNF)家族。
OX40 Ligand可以激活OX40，从而起到T细胞共刺激分子的作用。OX40-OX40配体相互作用促进效应T细胞存活并有效诱导记忆T细胞生成，增强Tfh对B细胞的辅助功能，并促进DC的分化和成熟^{[1]< /sup>[2]}。
小鼠 OX40 配体与大鼠的氨基酸序列一致性为 81.31%，与人类的氨基酸序列一致性<70%。
OX40 配体与 OX40 之间的相互作用对于抗原特异性记忆 T 细胞的生成至关重要，并诱导宿主抗肿瘤免疫^[4]。OX40 配体对于小鼠过敏性炎症的 Th1 和 Th2 反应至关重要^[5]。

OX4 Ligand (mouse, 1, 2 ng/mL) increases proliferation of CD4+ T cells isolated from mouse spleen^[6].

OX4 Ligand (mouse, 1 µg/kg, i.v.) exhibits higher eosinophil infiltration compared with control mice treated only with OVA in a Ovalbumin (OVA)‑induced asthma mouse model^[6].

Mouse

HEK293

N-rFc

P43488 (Q49-L198)

22164  [NCBI]

Approximately 44.4 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Kaur D, et al. OX40/OX40 ligand interactions in T-cell regulation and asthma. Chest. 2012 Feb;141(2):494-499.  [Content Brief]

  [2]. Fu N, et al. The OX40/OX40L Axis Regulates T Follicular Helper Cell Differentiation: Implications for Autoimmune Diseases. Front Immunol. 2021 Jun 21;12:670637.  [Content Brief]

  [3]. Croft M, et al. The significance of OX40 and OX40L to T-cell biology and immune disease. Immunol Rev. 2009 May;229(1):173-91.  [Content Brief]

  [4]. Buglio D, et al. HDAC11 plays an essential role in regulating OX40 ligand expression in Hodgkin lymphoma. Blood. 2011 Mar 10;117(10):2910-7  [Content Brief]

  [5]. Arestides RS, et al. Costimulatory molecule OX40L is critical for both Th1 and Th2 responses in allergic inflammation. Eur J Immunol. 2002 Oct;32(10):2874-80.  [Content Brief]

  [6]. Lei W, et al. Crucial role of OX40/OX40L signaling in a murine model of asthma. Mol Med Rep. 2018 Mar;17(3):4213-4220.  [Content Brief]