OX40/TNFRSF4 蛋白, Rhesus Macaque (HEK293, His)

OX40 (TNFRSF4), is a receptor for OX40 Ligand. OX40 is preferentially expressed by T cells. OX40 can be activated by OX40 Ligand, thereby functioning as a T cell co-stimulatory molecule. The OX40-OX40 Ligand interaction promotes effector T-cell survival and effectively induces memory T-cell generation, as well as enhances the helper function of Tfh for B cells, and also promotes the differentiation and maturation of DCs^[1][2]. OX40/TNFRSF4 Protein, Rhesus Macaque (HEK293, Fc) is a recombinant rhesus macaque OX40 (M1-A216) with C-terminal His tag, which is produced in HEK293.

OX40 (TNFRSF4) 是 TNFR 超家族的成员，是 OX40 配体的受体。OX40优先由T细胞表达，但也在自然杀伤T细胞、自然杀伤细胞、中性粒细胞和人气道平滑肌细胞中发现。人 OX40 与小鼠和大鼠的氨基酸序列同一性<30%。小鼠 OX40 与大鼠^[1]有 90% aa 序列同一性。
OX40 配体可以激活 OX40，从而起到 T 细胞共刺激分子的作用。OX40-OX40配体相互作用促进效应T细胞存活并有效诱导记忆T细胞生成，增强Tfh对B细胞的辅助功能，并促进DC的分化和成熟^{[1]< /sup>[2]}。
OX40配体与OX40之间的相互作用对于抗原特异性记忆T细胞的产生至关重要，并诱导宿主抗肿瘤免疫^[3]。但 OX40 和 OX40L 的过度上调可能会导致 Tfh 细胞异常激活和自身抗体过度产生，从而导致自身免疫性疾病^[1]。

OX4 increases expression of RANTES protein in HUVECs^[4].

Measured by its binding ability in a functional ELISA. When Recombinant Rhesus Macaque OX40 is immobilized at 2 μg/mL (100 μL/well), the concentration of Human OX40 Ligand that produces 50% of the optimal binding response is found to be approximately 1.369 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. When Recombinant Rhesus Macaque OX40 is immobilized at 2 μg/mL (100 μL/well), the concentration of Human OX40 Ligand that produces 50% of the optimal binding response is found to be approximately 1.369 ng/mL.

Rhesus Macaque

HEK293

C-6*His

XP_001090870.1 (K28-A216)

699674  [NCBI]

KLHCVGDTYPSNDRCCQECRPGNGMVSRCNRSQNTVCRPCGPGFYNDVVSAKPCKACTWCNLRSGSERKQPCTATQDTVCRCRAGTQPLDSYKPGVDCAPCPPGHFSPGDNQACKPWTNCTLAGKHTLQPASNSSDAICEDRDPPPTQPQETQGPPARPTTVQPTEAWPRTSQRPSTRPVEVPRGPAVA

Approximately 30-45 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kaur D, et al. OX40/OX40 ligand interactions in T-cell regulation and asthma. Chest. 2012 Feb;141(2):494-499.  [Content Brief]

  [2]. Fu N, et al. The OX40/OX40L Axis Regulates T Follicular Helper Cell Differentiation: Implications for Autoimmune Diseases. Front Immunol. 2021 Jun 21;12:670637.  [Content Brief]

  [3]. Buglio D, et al. HDAC11 plays an essential role in regulating OX40 ligand expression in Hodgkin lymphoma. Blood. 2011 Mar 10;117(10):2910-7.  [Content Brief]

  [4]. Kotani A, et al. Signaling of gp34 (OX40 ligand) induces vascular endothelial cells to produce a CC chemokine RANTES/CCL5. Immunol Lett. 2002 Oct 21;84(1):1-7.  [Content Brief]