P4HB 蛋白, Human (His)

The P4HB protein is a multifunctional entity that catalyzes disulfide bonding processes at the cell surface and within cells. As a reductase, it modifies outer surface proteins and participates in the formation of disulfide bonds in nascent proteins. P4HB Protein, Human (His) is the recombinant human-derived P4HB protein, expressed by E. coli , with N-6*His labeled tag. The total length of P4HB Protein, Human (His) is 490 a.a., with molecular weight of ~56 kDa.

P4HB 蛋白是一种多功能实体，可催化二硫键形成、断裂和重排的复杂过程。它位于细胞表面，充当还原酶，裂解与细胞相连的蛋白质的二硫键，并可能导致外表面蛋白质的结构修饰。在细胞内，P4HB 参与新生蛋白质二硫键的形成和重排。当浓度升高时，在 FAM20C 磷酸化的影响下，它充当伴侣，防止错误折叠的蛋白质聚集。相反，在较低浓度下，它通过促进聚集而表现出抗伴侣活性。P4HB 的多功能性延伸到它作为结构亚基参与多种酶，例如脯氨酰 4-羟化酶和微粒体三酰甘油转移蛋白 MTTP。此外，它作为 LGALS9 的受体，通过这种相互作用影响 Th2 T 辅助细胞表面的二硫键还原酶活性，改变质膜的氧化还原状态，并增强细胞迁移。这种多样化的功能强调了 P4HB 在细胞过程中的关键作用，包括蛋白质折叠、氧化还原调节和细胞迁移。

1.Thiol Protein Reductase Activity is 0.001 /650nm/ min^-2, determined by measuring the turbidityincrease at 650 nm due to insulin reduction.The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag tim.
2. Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is 9.375 A650/cm/min/mg.

Human

E. coli

N-6*His

P07237 (A19-L508)

5034  [NCBI]

APEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL

Approximately 56 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.