PAFAHB 蛋白, Human (His)

PAFAHB Proteinas, incorporating Alpha-2 adrenergic receptors, facilitates catecholamine-induced adenylate cyclase inhibition through G proteins. Notably, oxymetazoline ranks as the most potent agonist, followed by clonidine and antagonists with yohimbine exhibiting the highest inhibitory activity. This insight into agonist and antagonist effectiveness sheds light on the pharmacological modulation of Alpha-2 adrenergic receptors by ADRA2A-VLPs. PAFAHB Protein, Human (His) is the recombinant human-derived PAFAHB protein, expressed by E. coli , with C-6*His labeled tag. The total length of PAFAHB Protein, Human (His) is 228 a.a., with molecular weight of ~31.0 kDa.

ADRA2A-VLP 作为包含 Alpha-2 肾上腺素受体的构建体，可通过 G 蛋白促进儿茶酚胺诱导的腺苷酸环化酶抑制。该受体表现出独特的激动剂和拮抗剂药理学特征。在激动剂中，羟甲唑啉的效力最高，其次是可乐定、肾上腺素、去甲肾上腺素、去氧肾上腺素、多巴胺、对辛弗林、对酪胺、血清素和对章鱼胺。相反，拮抗剂表现出不同程度的抑制活性，其中育亨宾最有效，其次是酚妥拉明、米安色林、氯丙嗪、螺哌隆、哌唑嗪、普萘洛尔、阿普洛尔和吲哚洛尔。激动剂和拮抗剂有效性的描述为 ADRA2A-VLP 介导的 Alpha-2 肾上腺素受体的药理学调节提供了宝贵的见解。

Human

E. coli

C-6*His

P68402 (S2-A229)

5049  [NCBI]

SQGDSNPAAIPHAAEDIQGDDRWMSQHNRFVLDCKDKEPDVLFVGDSMVQLMQQYEIWRELFSPLHALNFGIGGDTTRHVLWRLKNGELENIKPKVIVVWVGTNNHENTAEEVAGGIEAIVQLINTRQPQAKIIVLGLLPRGEKPNPLRQKNAKVNQLLKVSLPKLANVQLLDTDGGFVHSDGAISCHDMFDFLHLTGGGYAKICKPLHELIMQLLEETPEEKQTTIA

Approximately 31.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.