PAM 蛋白, Human (HEK293, His)

The PAM protein is a bifunctional enzyme that coordinates the α-amidation process, which is critical for the biosynthesis of neuropeptides and endocrine peptides. The peptidyl α-hydroxylating monooxygenase (PHM) domain hydroxylates the C-terminal glycine, and the peptidylglycine amide glycolate lyase (PAL) domain cleaves the NC-α bond, producing α-amidated peptides. PAM Protein, Human (HEK293, His) is the recombinant human-derived PAM protein, expressed by HEK293 , with C-His labeled tag.

PAM 蛋白作为一种双功能酶，负责监督无活性肽基甘氨酸前体对其生物活性 α-酰胺化肽形式的翻译后修饰，这是众多神经和内分泌肽生物合成中的关键末端修饰。α-酰胺化过程涉及两个连续反应，每个反应均由酶内不同的催化域控制。第一步，肽基 α-羟基化单加氧酶 (PHM) 结构域在 C 末端甘氨酸的 α-碳 (C-α) 处催化铜、抗坏血酸和 O2 依赖性立体特异性羟基化（具有 S 立体化学）肽基甘氨酸底物。随后的步骤由肽基甘氨酸酰胺乙醇酸裂解酶 (PAL) 结构域精心策划，需要锌依赖性 NC-α 键裂解，从而产生 α-酰胺化肽和乙醛酸。此外，PAM 表现出类似的催化 N-脂肪酰基甘氨酸两步转化为伯脂肪酸酰胺和乙醛酸盐的能力，其方式让人想起其肽基甘氨酸修饰功能。

Measured by its ability to convert Hippurate to Benzamide and Glyoxylate. The specific activity is 731.87 pmol/min/μg, as measured under the described conditions.

Human

HEK293

C-His

P19021-2/NP_620176.1 (F21-S866)

5066  [NCBI]

Approximately 94.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.