1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PNLIP/Pancreatic lipase 蛋白, Mouse (His-SUMO)

PNLIP/Pancreatic lipase 蛋白, Mouse (His-SUMO)

目录号: HY-P71582
产品使用指南

胰脂肪酶 (PNLIP) 通过酶促分解膳食脂质并优先选择 1 和 3 位的长链脂肪酸酯,在脂肪代谢中发挥着至关重要的作用。这导致主要产生 2-单酰基甘油和游离脂肪酸。PNLIP/Pancreatic lipase 蛋白, Mouse (His-SUMO) 是重组的 PNLIP/胰脂肪酶蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。

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描述

Pancreatic lipase (PNLIP) plays a crucial role in fat metabolism by enzymatically breaking down dietary lipids and preferentially selecting long-chain fatty acid esters at positions 1 and 3. This results in the production of mainly 2-monoacylglycerol and free fatty acids. PNLIP/Pancreatic lipase Protein, Mouse (His-SUMO) is the recombinant mouse-derived PNLIP/Pancreatic lipase protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

研究背景

胰脂肪酶 (PNLIP) 蛋白是脂肪代谢不可或缺的一部分,在膳食脂质的酶促分解中发挥着至关重要的作用。PNLIP 显示出在位置 1 和 3 处裂解长链脂肪酸的酯的偏好,导致主要产生 2-单酰基甘油和游离脂肪酸。值得注意的是,与可溶性乳化底物相比,其对不溶性乳化底物的酶活性显着更高。这种底物特异性强调了蛋白质在处理膳食脂肪和促进消化系统中脂质的消化和吸收方面的效率。PNLIP 的功能特征凸显了其在脂质代谢中的重要参与,在促进利用膳食脂肪进行能量和营养吸收的复杂过程中发挥着关键作用。

种属

Mouse

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

Q6P8U6 (R17-C465)

基因 ID
蛋白结构
N-term
6*His-SUMO
PNLIP (R17-C465)
Accession # Q6P8U6
C-term
同用名
PnlipPancreatic triacylglycerol lipase; PL; PTL; Pancreatic lipase; EC 3.1.1.3
氨基酸序列

REVCFDKLGCFSDDAPWSGTLDRPLKALPWSPAQINTRFLLYTNENPDNYQLITSDASNIRNSNFRTNRKTRIIIHGFIDKGEENWLSDMCKNMFRVESVNCICVDWKGGSRTTYTQATQNVRVVGAEVALLVNVLQSDLGYSLNNVHLIGHSLGSHIAGEAGKRTFGAIGRITGLDPAEPYFQGTPEEVRLDPTDAQFVDAIHTDAGPIIPNLGFGMSQTVGHLDFFPNGGIEMPGCQKNILSQIVDIDGIWEGTRNFAACNHLRSYKFYTDSIVNPTGFAGFSCSSYSLFTANKCFPCGSGGCPQMGHYADRYPGKTSRLYQTFYLNTGDKSNFARWRYQVTVTLSGQKVTGHILVSLFGNGGNSKQYEVFKGSLQPGTSHVNEFDSDVDVGDLQKVKFIWYNNVINPTLPKVGASRITVERNDGRVFNFCSQETVREDVLLTLSPC

分子量

Approximately 65.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PNLIP/Pancreatic lipase Protein, Mouse (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PNLIP/Pancreatic lipase Protein, Mouse (His-SUMO)
目录号:
HY-P71582
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